中华内分泌代谢杂志
中華內分泌代謝雜誌
중화내분비대사잡지
CHINESE JOURNAL OF ENDOCRINOLOGY AND METABOLISM
2010年
5期
413-415
,共3页
李冰%杨菊红%王楠%梁东春%江昌新%冯凭
李冰%楊菊紅%王楠%樑東春%江昌新%馮憑
리빙%양국홍%왕남%량동춘%강창신%풍빙
OLETF%Chemefin%CMKLR1%实时PCR%基因表达
OLETF%Chemefin%CMKLR1%實時PCR%基因錶達
OLETF%Chemefin%CMKLR1%실시PCR%기인표체
OLETF%Chemerin%CMKLR1%Real-time PCR%Gene expression
用实时定量PCR检测OLETF大鼠脂肪组织中chemerin及其受体1 mRNA的表达,结果两者表达水平较对照组明显升高(P<0.05或P<0.01),且网膜脂肪组织高于皮下脂肪组织(P<0.05或P<0.01),说明脂肪因子chemerin及其受体1基因表达的变化可能是肥胖、胰岛素抵抗和2型糖尿病发病的机制之一.
用實時定量PCR檢測OLETF大鼠脂肪組織中chemerin及其受體1 mRNA的錶達,結果兩者錶達水平較對照組明顯升高(P<0.05或P<0.01),且網膜脂肪組織高于皮下脂肪組織(P<0.05或P<0.01),說明脂肪因子chemerin及其受體1基因錶達的變化可能是肥胖、胰島素牴抗和2型糖尿病髮病的機製之一.
용실시정량PCR검측OLETF대서지방조직중chemerin급기수체1 mRNA적표체,결과량자표체수평교대조조명현승고(P<0.05혹P<0.01),차망막지방조직고우피하지방조직(P<0.05혹P<0.01),설명지방인자chemerin급기수체1기인표체적변화가능시비반、이도소저항화2형당뇨병발병적궤제지일.
The mRNA expressions of chemerin and its receptor CMKLR1 in adipose tissue of OLETF rats and LETO rats were detected by real-time PCR. The results showed that the two genes mRNA expressions in subcutaneous and visceral adipose tissue in OLETF group were significantly higher than those in LETO group (P< 0.05 or P<0.01) and expressions of them in visceral adipose tissue were higher than those in subcutaneous adipose tissue(P<0. 05 or P<0. 01), suggesting that chemerin and CMKLR1 may be involved in the pathogenesis of obesity, insulin resistance, and type 2 diabetes mellitus.
