大鼠脑内血肿继发性损伤7T核磁共振影像学研究
대서뇌내혈종계발성손상7T핵자공진영상학연구
Secondary injury in rats with intra-cerebral hematoma: a 7 Tesla high-field strength MRI study
  • 万方数据
    中华神经医学杂志 중화신경의학잡지
    CHINESE JOURNAL OF NEUROMEDICINE
    2012年 6期 586-589 ,共4页

    张俊海%顾建文%郜发宝%夏睿%杨涛%匡永勤%夏勋%马原%程敬民%孔滨%黄海东%曾凡俊%卢敏%冉春梅%李运明

    장준해%고건문%고발보%하예%양도%광영근%하훈%마원%정경민%공빈%황해동%증범준%로민%염춘매%리운명

    颅内血肿%继发性损伤%磁共振成像%铁离子%动物模型 顱內血腫%繼髮性損傷%磁共振成像%鐵離子%動物模型
    로내혈종%계발성손상%자공진성상%철리자%동물모형
    Intra-cerebral hematoma%Secondary injury%Magnetic resonance imaging%Iron%Animal modeling
    目的 应用高场强7T核磁共振影像仪动态分析脑出血大鼠脑水肿吸收演化过程及局部脑铁含量演变情况,探讨脑出血后继发性损伤病程特点及机制. 方法 SD成年大鼠16只,按随机数字表法分为实验组(n=10)和对照组(n=6).实验组右侧尾状核注入自体静脉血50 μL,对照组同部位等量注入生理盐水.建模后1、2、3、7及14d对2组大鼠分别行7T头颅核磁共振成像T2及T2-star序列扫描,对比分析影像学变化特点. 结果 实验组成功建模9只,术毕死亡1只.早期(3 d内)T2加权成像清晰,血肿周边T2弛豫时间上升迅速,3d后逐渐平缓,7d达高峰;建模后损害范围吸收减小,3d时转而出现扩大趋势,7d左右达峰值.建模3d后T2-star值迅速出现峰值,后缓和下降.对照组除针道损伤外,未见其他明显信号改变. 结论 脑出血后继发性损伤呈现短期内快速进展,平稳后再次加剧的演变趋势,病灶局部铁含量变化趋势与继发性损伤变化趋势同步,提示铁在继发性脑损伤发生机制中起重要作用.
    목적 응용고장강7T핵자공진영상의동태분석뇌출혈대서뇌수종흡수연화과정급국부뇌철함량연변정황,탐토뇌출혈후계발성손상병정특점급궤제. 방법 SD성년대서16지,안수궤수자표법분위실험조(n=10)화대조조(n=6).실험조우측미상핵주입자체정맥혈50 μL,대조조동부위등량주입생리염수.건모후1、2、3、7급14d대2조대서분별행7T두로핵자공진성상T2급T2-star서렬소묘,대비분석영상학변화특점. 결과 실험조성공건모9지,술필사망1지.조기(3 d내)T2가권성상청석,혈종주변T2이예시간상승신속,3d후축점평완,7d체고봉;건모후손해범위흡수감소,3d시전이출현확대추세,7d좌우체봉치.건모3d후T2-star치신속출현봉치,후완화하강.대조조제침도손상외,미견기타명현신호개변. 결론 뇌출혈후계발성손상정현단기내쾌속진전,평은후재차가극적연변추세,병조국부철함량변화추세여계발성손상변화추세동보,제시철재계발성뇌손상발생궤제중기중요작용.
    Objective To dynamically analyze the evolutionary process of cerebral edema absorption and the level of local iron in rats with intra-cerebral hematoma by high-field strength 7 Tesla MRI and explore the characteristics and mechanism of secondary injury after intra-cerebral hematoma.Methods Sixteen adult SD rats (about 150 g) were randomly divided into experimental group (n=10) and control group (n=6).Rat models in the experimental group were established by performing injection of 50 μL their own venous blood into their right caudate nucleus accurately. Rats in the control group were used normal saline,instead.After that,head MRI (T2 and T2-star scans) was performed 1,2,3,7 and 14 d after the injection; their imaging features were compared. Results Nine rats in the experimental group survived and 1 died after the operation; in the early days (within 3 d), the T2 weighing imaging showed that the time of relaxation surrounding the hematoma was longer than that in control group,suggesting that the zone of the edema surrounding the hematoma became more clearly.In the early days (within 3 d),T2-weighted imaging was clear,and the time of relaxation surrounding the hematoma increased rapidly,steadily improved 3 d after the operation and reached its peak level 7 dafter the operation; the damage area absorption decreased steadily but turned widening 3 d later and reached the peak 7 d later.T2-star value reached the peak rapidly 3 d after the operation,and then,moderated the downturn.The rats in the control group showed no obvious signal changes under MRI,except those with needle tract injury. Conclusion Secondary injury after intra-cerebral hemorrhage shows a rapidly injury progress in the short terrn at first,and then,has intensify again after a stable period; the local iron diffusion trend is synchronized to the secondary injury,suggesting that iron may play a key role in the mechanism of secondary brain edema.
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