中华眼科杂志
中華眼科雜誌
중화안과잡지
Chinese Journal of Ophthalmology
2010年
2期
140-144
,共5页
萄糖醛酸糖苷酸%缺氧诱导因子1,α亚基%血管内皮生长因子A%视网膜母细胞瘤%逆转录聚合酶链反应%免疫组织化学
萄糖醛痠糖苷痠%缺氧誘導因子1,α亞基%血管內皮生長因子A%視網膜母細胞瘤%逆轉錄聚閤酶鏈反應%免疫組織化學
도당철산당감산%결양유도인자1,α아기%혈관내피생장인자A%시망막모세포류%역전록취합매련반응%면역조직화학
Glucuronidase%Hypoxia-inducible factor1,alpha subunit%Vascular endothelial growth factor A%Retinoblastoma%Reverse transcriptase polymerase chain reaction%Immunohistochemistry
目的 探讨视网膜母细胞瘤(RB)中乙酰肝素酶(HPSE)、低氧诱导因子-1α(HIF-1α)的表达及其与血管内皮生长因子(VEGF)、微血管密度(MVD)的关系.方法 应用免疫组织化学法检测34例RB石蜡标本及10例正常视网膜组织中HIF-1α、VEGF蛋白表达,用CD34抗体进行肿瘤血管内皮染色,计数肿瘤微血管密度(MVD);应用RT-PCR检测18例RB组织中 HPSE mRNA、HIF-1α mRNA、VEGF mRNA的表达,分析RB组织中HPSE、HIF-1α、VEGF表达情况及其与肿瘤微血管形成、临床及病理特征的关系.计量资料采用成组设计定量资料t检验、方差分析和q检验;计数资料采用χ~2检验、Fisher检验及秩和检验;相关性采用Spearman等级相关分析及kappa检验.结果 34例RB石蜡标本中HIF-1α、VEGF阳性表达率为58.8%、64.7%,明显高于正常对照组(χ~2=10.784,P<0.05;χ~2=9.269,P<0.05).18例RB中HPSE、HIF-1α、VEGF mRNA阳性率为55.6%、44.4%、72.2%,与正常组比较有统计学意义(χ~2=8.642,P<0.05;χ~2=6.222,P<0.05;χ~2=9.956,P<0.05).HIF-1α、VEGF与MVD的表达呈正相关(r=0.664,P<0.05;r=0.590,P<0.05);RB中HIF-1α mRNA、HPSE mRNA表达与VEGF mRNA表达呈相关性(Z=2.350,P=0.009;Z=2.940,P=0.002).结论 HIF-1α、HPSE可共同作用于VEGF这种促血管生成重要因子,在肿瘤血管形成中起一定作用.
目的 探討視網膜母細胞瘤(RB)中乙酰肝素酶(HPSE)、低氧誘導因子-1α(HIF-1α)的錶達及其與血管內皮生長因子(VEGF)、微血管密度(MVD)的關繫.方法 應用免疫組織化學法檢測34例RB石蠟標本及10例正常視網膜組織中HIF-1α、VEGF蛋白錶達,用CD34抗體進行腫瘤血管內皮染色,計數腫瘤微血管密度(MVD);應用RT-PCR檢測18例RB組織中 HPSE mRNA、HIF-1α mRNA、VEGF mRNA的錶達,分析RB組織中HPSE、HIF-1α、VEGF錶達情況及其與腫瘤微血管形成、臨床及病理特徵的關繫.計量資料採用成組設計定量資料t檢驗、方差分析和q檢驗;計數資料採用χ~2檢驗、Fisher檢驗及秩和檢驗;相關性採用Spearman等級相關分析及kappa檢驗.結果 34例RB石蠟標本中HIF-1α、VEGF暘性錶達率為58.8%、64.7%,明顯高于正常對照組(χ~2=10.784,P<0.05;χ~2=9.269,P<0.05).18例RB中HPSE、HIF-1α、VEGF mRNA暘性率為55.6%、44.4%、72.2%,與正常組比較有統計學意義(χ~2=8.642,P<0.05;χ~2=6.222,P<0.05;χ~2=9.956,P<0.05).HIF-1α、VEGF與MVD的錶達呈正相關(r=0.664,P<0.05;r=0.590,P<0.05);RB中HIF-1α mRNA、HPSE mRNA錶達與VEGF mRNA錶達呈相關性(Z=2.350,P=0.009;Z=2.940,P=0.002).結論 HIF-1α、HPSE可共同作用于VEGF這種促血管生成重要因子,在腫瘤血管形成中起一定作用.
목적 탐토시망막모세포류(RB)중을선간소매(HPSE)、저양유도인자-1α(HIF-1α)적표체급기여혈관내피생장인자(VEGF)、미혈관밀도(MVD)적관계.방법 응용면역조직화학법검측34례RB석사표본급10례정상시망막조직중HIF-1α、VEGF단백표체,용CD34항체진행종류혈관내피염색,계수종류미혈관밀도(MVD);응용RT-PCR검측18례RB조직중 HPSE mRNA、HIF-1α mRNA、VEGF mRNA적표체,분석RB조직중HPSE、HIF-1α、VEGF표체정황급기여종류미혈관형성、림상급병리특정적관계.계량자료채용성조설계정량자료t검험、방차분석화q검험;계수자료채용χ~2검험、Fisher검험급질화검험;상관성채용Spearman등급상관분석급kappa검험.결과 34례RB석사표본중HIF-1α、VEGF양성표체솔위58.8%、64.7%,명현고우정상대조조(χ~2=10.784,P<0.05;χ~2=9.269,P<0.05).18례RB중HPSE、HIF-1α、VEGF mRNA양성솔위55.6%、44.4%、72.2%,여정상조비교유통계학의의(χ~2=8.642,P<0.05;χ~2=6.222,P<0.05;χ~2=9.956,P<0.05).HIF-1α、VEGF여MVD적표체정정상관(r=0.664,P<0.05;r=0.590,P<0.05);RB중HIF-1α mRNA、HPSE mRNA표체여VEGF mRNA표체정상관성(Z=2.350,P=0.009;Z=2.940,P=0.002).결론 HIF-1α、HPSE가공동작용우VEGF저충촉혈관생성중요인자,재종류혈관형성중기일정작용.
Objective To investigate the expression of heparanase (HPSE),hypoxia-inducible factor (HIF-1α)and their correlation with expression of vascular endothelial growth factor (VEGF) and microvessel density (MVD) in retinoblastoma.Methods HIF-1αand VEGF were detected by SP immunohistochemical method in 34 cases of retinoblagtoma and 10 cases of normal retina tissues.MVD was measured by anti-CD34.Eighteen samples of retinoblastoma tissues and ten samples of normal retina were examined for HPSE mRNA,HIF-1αmRNA,VEGF mRNA expression by RT-PCR.The correlation between HPSE,HIF-1α and VEGF expression,MVD and clinical and pathological characters were analyzed.The statistical methods are the measurement data using grouped quantitative data t test,variance analysis and q test;enumeration data compared with χ~2 test,Fisher test and rank sum test;correlation analysis using Spearman rank correlaion analysis and the kappa test.Results The positive expression rates of HIF-1α and VEGF were 58.8%,64.7%higher in retinoblastoma than in normal retina's(χ~2=10.784,P<0.05; χ~2=9.269,P<0.05).Positive expression of HPSE、HIF-1α、VEGF mRNA were bund in 55.6%、44.4%、72.2%.The expression of HIF-1α and VEGF in retinoblastoma were correlated with MVD(r=0.664,P<0.05;r=0.590,P<0.05).VEGF wag correlated with HPSE and HIF-1α(Z=2.350,P=0.009;Z=2.940.P=0.002).Conclusions HPSE and HIF-1α can influenee the expression of VEGF,an important angiogenesis factor.HPSE,HIF-1α and VEGF phay a role in tumor angiogenesis and promote malignant progress of retinoblastoma.
