肿瘤研究与临床
腫瘤研究與臨床
종류연구여림상
CANCER RESEARCH AND CLINIC
2009年
5期
295-297
,共3页
刘静%邢欣%邢凌霄%周炳娟%严霞%王俊灵%李月红%张祥宏
劉靜%邢訢%邢凌霄%週炳娟%嚴霞%王俊靈%李月紅%張祥宏
류정%형흔%형릉소%주병연%엄하%왕준령%리월홍%장상굉
胃肿瘤%脱氧雪腐镰刀菌烯醇%SNU细胞%细胞凋亡
胃腫瘤%脫氧雪腐鐮刀菌烯醇%SNU細胞%細胞凋亡
위종류%탈양설부렴도균희순%SNU세포%세포조망
Stomach neoplasms%Deoxynivalenol (DON)%SNU cells%Apoptosis
目的 探讨脱氧雪腐镰刀菌烯醇(DON)对胃癌细胞SNU凋亡的影响.方法 体外培养的胃癌细胞株SNU经不同浓度(50、100、1000、2000 μg/L)DON处理12 h,以流式细胞术(FCM)和免疫蛋白印记(Westem blotting)法检测细胞凋亡和凋亡相关蛋白bax、bcl-2、Caspase-3的表达情况.结果 FCM检测结果表明,DON处理12 h后,各处理组SNU细胞凋亡率均高于对照组.在50～2000 μg/L浓度范围内随着DON浓度升高凋亡率亦相应升高,两者呈明显剂量依赖关系(r=0.940,P<0.01).FCM和WPstPm blotting法检测结果显示,DON作用12 h后,SNU细胞bax和Caspase-3表达升高,bcl-2表达降低.结论 DON可剂量依赖地诱导体外培养的胃癌细胞SNU凋亡,上调bax蛋白的表达,下凋bcl-2蛋白的表达,进而激活Caspaqe-3,可能在SUN细胞凋亡过程中发挥作用.
目的 探討脫氧雪腐鐮刀菌烯醇(DON)對胃癌細胞SNU凋亡的影響.方法 體外培養的胃癌細胞株SNU經不同濃度(50、100、1000、2000 μg/L)DON處理12 h,以流式細胞術(FCM)和免疫蛋白印記(Westem blotting)法檢測細胞凋亡和凋亡相關蛋白bax、bcl-2、Caspase-3的錶達情況.結果 FCM檢測結果錶明,DON處理12 h後,各處理組SNU細胞凋亡率均高于對照組.在50～2000 μg/L濃度範圍內隨著DON濃度升高凋亡率亦相應升高,兩者呈明顯劑量依賴關繫(r=0.940,P<0.01).FCM和WPstPm blotting法檢測結果顯示,DON作用12 h後,SNU細胞bax和Caspase-3錶達升高,bcl-2錶達降低.結論 DON可劑量依賴地誘導體外培養的胃癌細胞SNU凋亡,上調bax蛋白的錶達,下凋bcl-2蛋白的錶達,進而激活Caspaqe-3,可能在SUN細胞凋亡過程中髮揮作用.
목적 탐토탈양설부렴도균희순(DON)대위암세포SNU조망적영향.방법 체외배양적위암세포주SNU경불동농도(50、100、1000、2000 μg/L)DON처리12 h,이류식세포술(FCM)화면역단백인기(Westem blotting)법검측세포조망화조망상관단백bax、bcl-2、Caspase-3적표체정황.결과 FCM검측결과표명,DON처리12 h후,각처리조SNU세포조망솔균고우대조조.재50～2000 μg/L농도범위내수착DON농도승고조망솔역상응승고,량자정명현제량의뢰관계(r=0.940,P<0.01).FCM화WPstPm blotting법검측결과현시,DON작용12 h후,SNU세포bax화Caspase-3표체승고,bcl-2표체강저.결론 DON가제량의뢰지유도체외배양적위암세포SNU조망,상조bax단백적표체,하조bcl-2단백적표체,진이격활Caspaqe-3,가능재SUN세포조망과정중발휘작용.
Objective To explore the effects of deoxynivalenol (DON) on apoptosis of human gastric carcinoma cell line SNU in vitro. Methods SNU cells were treated with DON at different concentrations (50, 100, 1000, 2000 μg/L) for 12 hours, and then cells were harvested for cell apoptosis by flow cytometric (FCM) DNA analysis and the expression of Bax, Bcl-2 and Caspase-3 at protein level with FCM and Western blotting. Results FCM results showed that the apoptosis rates of SNU cells in DON treatment groups were all higher than that in control, especially in DON 1000 μg/L and 2000 μg/L groups (P<0.05). In the concentration range from 50 to 2000 μg/L, a significant concentration-depended response correlation could be found between apoptosis rate and DON concentration (r =0.940, P <0.01). FCM and Western blotting showed Bax and Caspase-3 expression in often SNU cells DON treatment for 12 hours were up-regulated while that of Bcl-2 was down-regulated. Conclusion DON can induce apoptosis of SNU cells in vitro in dose-dependent manner, and possible mechanisms of apoptosis induction effects may be up-regulation of the expression of Bax and down-regulation of that of Bcl-2 and activation of the key enzyme of apoptosis Caspase-3.
