CAJ | 학술논문

通过建立慢性豚鼠哮喘模型,研究重组人磷脂酶D2(rhPLD2)干预慢性哮喘豚鼠血清中糖基化磷脂酰肌醇特异性磷脂酶D(GPI-PLD)含量的变化,探寻可能的机制.以rhPLD2干预慢性哮喘豚鼠.用TX-114分相法检测豚鼠血清中GPI-PLD酶活性.与正常状态豚鼠相比较,慢性哮喘状态豚鼠其血清中GPI-PLD酶活性显著升高.但以rhPLD2及地塞米松干预慢性哮喘豚鼠后,该指标显著下降.rhPLD2可通过抑制其血清中GPI-PLD酶活性表达,来抑制哮喘发生过程中的炎症反应.
통과건립만성돈서효천모형,연구중조인린지매D2(rhPLD2)간예만성효천돈서혈청중당기화린지선기순특이성린지매D(GPI-PLD)함량적변화,탐심가능적궤제.이rhPLD2간예만성효천돈서.용TX-114분상법검측돈서혈청중GPI-PLD매활성.여정상상태돈서상비교,만성효천상태돈서기혈청중GPI-PLD매활성현저승고.단이rhPLD2급지새미송간예만성효천돈서후,해지표현저하강.rhPLD2가통과억제기혈청중GPI-PLD매활성표체,래억제효천발생과정중적염증반응.
The effect of recombinant human phospholipase D2(rhPLD2)in vivo was investigated on the secretion of serum glycosyl phosphatidylinositol-specific phospholipase D(GPI-PLD)in guinea pigs of chronic asthma．Ater treating the guinea pigs attacked by chronic asthma with rhPLD2,the GPI-PLD activity detection was canrried out by phase separation of human placental alkaline phosphatase in Triton X-114．Compared with the healthy guinea pigs(NS group),the serum GPI-PLD in the guinea pigs of chronic asthma are much higher than that of control groups,P≤0．01．Our results showed that rhPLD2 could significantly reduce the secretion of GPI-PLD when the guinea pigs were attacked by chronic asthma．