CAJ | 학술논문

目的研究黄芪多糖对小鼠免疫功能的影响.方法采用ConA诱导小鼠淋巴细胞转化试验及迟发性变态反应试验测试黄芪多糖(Astragalus Polysaccharide APS)对小鼠细胞免疫功能的影响;采用抗体生成细胞检测及半数溶血值实验测试黄芪多糖对小鼠体液免疫的影响;采用小鼠腹腔巨噬细胞吞噬鸡红细胞试验测试黄芪多糖对小鼠非特异性免疫的影响.结果黄芪多糖能明显提高小鼠的足跖肿胀度、脾脏生成抗体细胞数、半数溶血值及巨噬细胞吞噬功能,黄芪多糖对小鼠淋巴细胞增殖能力无明显影响.结论黄芪多糖对正常小鼠的细胞免疫、体液免疫及巨噬细胞功能均有明显的增强作用.
목적 연구황기다당대소서면역공능적영향.방법 채용ConA유도소서림파세포전화시험급지발성변태반응시험측시황기다당(Astragalus Polysaccharide APS)대소서세포면역공능적영향;채용항체생성세포검측급반수용혈치실험측시황기다당대소서체액면역적영향;채용소서복강거서세포탄서계홍세포시험측시황기다당대소서비특이성면역적영향.결과 황기다당능명현제고소서적족척종창도、비장생성항체세포수、반수용혈치급거서세포탄서공능,황기다당대소서림파세포증식능력무명현영향.결론 황기다당대정상소서적세포면역、체액면역급거서세포공능균유명현적증강작용.
Objective To investigate the effect of astragalus polysaccharide (APS) on immunological function in mice.Methods By lymphocyte transformation test and delayed type hypersensitivity (DTH),the effect of APS on celluar immunity was evaluated.By determining plaque forming cells (PFC) and HC50,the effect of APS on humoral immunity was studied.Abdomen macrophages of mice devour chicken red cell was used to asscess phagositosis of peritoneal macrophages.Results Compared with the normal control group,APS could promote DTH,PFC,HC50 and phagositosis of peritoneal macrophages,but had no effect on lymphocyte proliferation.Conclusion APS can stimulate the immunological function in mice in different ways.