CAJ | 학술논문

目的建立稳定的大鼠非套管法异体原位左肺移植模型.方法使用250～350 g Sprague Dawley (SD)大鼠进行实验,采用显微外科技术,分别连续缝合支气管和肺动、静脉,建立大鼠异体原位左肺移植模型.观察大鼠存活时间,移植肺脏血管、支气管吻合口通畅性.结果 10只接受左肺移植的受体大鼠均成功脱离呼吸机,供肺冷缺血时间约40 min,热缺血时间约40 min,总手术时间约130 min.解剖后检查示各吻合口均通畅.结论非套管法大鼠原位左肺移植模型具有稳定、可靠、接近临床肺移植的优点.
목적 건립은정적대서비투관법이체원위좌폐이식모형.방법 사용250～350 g Sprague Dawley (SD)대서진행실험,채용현미외과기술,분별련속봉합지기관화폐동、정맥,건립대서이체원위좌폐이식모형.관찰대서존활시간,이식폐장혈관、지기관문합구통창성.결과 10지접수좌폐이식적수체대서균성공탈리호흡궤,공폐랭결혈시간약40 min,열결혈시간약40 min,총수술시간약130 min.해부후검사시각문합구균통창.결론 비투관법대서원위좌폐이식모형구유은정、가고、접근림상폐이식적우점.
Objective To establish a stable model of rat orthotopic left lung transplantation using direct suture of vessels and bronchi. Methods Ten Sprague Dawley (SD) rats weighted 250 to 350 g were used as lung donors and recipients respectively. Airway and pulmonary vessels were reconstructed microsurgically using continuous running suture technique. Survival time were recorded and donor lungs were checked by autopsy. Results All 10 rats received left lung transplantation were weaned from ventilator successfully. Both of cold ischemia time and warm ischemia time were about 40 minutes. The total procedure took about 130 minutes. Autopsy was used to check the patency of anastomotic sites. No thrombosis or air leak was found. Conclusions Direct microsurgical surture can be used to establish an experimental model of orthotopic left allograft lung transplantation in rats. This method is proved to be stable, reliable and similar to clinical practices.