作物学报
作物學報
작물학보
ACTA AGRONOMICA SINICA
2010年
4期
629-635
,共7页
王旺田%张金文%王蒂%陶士珩%季彦林%吴兵
王旺田%張金文%王蒂%陶士珩%季彥林%吳兵
왕왕전%장금문%왕체%도사형%계언림%오병
马铃薯%光质%信号转导分子%糖苷生物碱
馬鈴藷%光質%信號轉導分子%糖苷生物堿
마령서%광질%신호전도분자%당감생물감
Potato%Light qualities%Signal transduction molecule%Steroidal glycoalkaloids
马铃薯块茎糖苷生物碱(SGAs)含量如果超过20 mg 100 g~(-1) FW,人畜食用即可引起中毒.而马铃薯块茎中SGAs的积累受多种因因素的影响,其中受温度与光照影响较大.为减少马铃薯块茎中SGAs的积累,了解SGAs在马铃薯块茎中的积累机制,研究了同一温度下不同光质对马铃薯块茎SGAs的积累的影响.结果表明红光照射后各品种SGAs平均含量比蓝光、白光、黑暗处理分别提高26.02%、55.50%和100.79%,且在α=0.01水平上差异极显著,说明不同光质对马铃薯块茎SGAs含量影响不同,红光影响最大.蓝光次之.同时,马铃薯块茎经不同光质处理后作为第二信使的G蛋向含量在红光下较蓝光、黑暗、白光下分别上升0.95%、2.01%、3.86%,钙调蛋白含量分别增加7.94%、37.41%和87.24%.由此可知,红光是SGAs积累的重要信号分子,其信号作用启动了红光受体PHYB,与第二信使G蛋门和CaM等共同参与马铃薯块茎SGAs的代谢积累.
馬鈴藷塊莖糖苷生物堿(SGAs)含量如果超過20 mg 100 g~(-1) FW,人畜食用即可引起中毒.而馬鈴藷塊莖中SGAs的積纍受多種因因素的影響,其中受溫度與光照影響較大.為減少馬鈴藷塊莖中SGAs的積纍,瞭解SGAs在馬鈴藷塊莖中的積纍機製,研究瞭同一溫度下不同光質對馬鈴藷塊莖SGAs的積纍的影響.結果錶明紅光照射後各品種SGAs平均含量比藍光、白光、黑暗處理分彆提高26.02%、55.50%和100.79%,且在α=0.01水平上差異極顯著,說明不同光質對馬鈴藷塊莖SGAs含量影響不同,紅光影響最大.藍光次之.同時,馬鈴藷塊莖經不同光質處理後作為第二信使的G蛋嚮含量在紅光下較藍光、黑暗、白光下分彆上升0.95%、2.01%、3.86%,鈣調蛋白含量分彆增加7.94%、37.41%和87.24%.由此可知,紅光是SGAs積纍的重要信號分子,其信號作用啟動瞭紅光受體PHYB,與第二信使G蛋門和CaM等共同參與馬鈴藷塊莖SGAs的代謝積纍.
마령서괴경당감생물감(SGAs)함량여과초과20 mg 100 g~(-1) FW,인축식용즉가인기중독.이마령서괴경중SGAs적적루수다충인인소적영향,기중수온도여광조영향교대.위감소마령서괴경중SGAs적적루,료해SGAs재마령서괴경중적적루궤제,연구료동일온도하불동광질대마령서괴경SGAs적적루적영향.결과표명홍광조사후각품충SGAs평균함량비람광、백광、흑암처리분별제고26.02%、55.50%화100.79%,차재α=0.01수평상차이겁현저,설명불동광질대마령서괴경SGAs함량영향불동,홍광영향최대.람광차지.동시,마령서괴경경불동광질처리후작위제이신사적G단향함량재홍광하교람광、흑암、백광하분별상승0.95%、2.01%、3.86%,개조단백함량분별증가7.94%、37.41%화87.24%.유차가지,홍광시SGAs적루적중요신호분자,기신호작용계동료홍광수체PHYB,여제이신사G단문화CaM등공동삼여마령서괴경SGAs적대사적루.
The potato steroidal glycoalkaloids (SGAs) are secondary metabolites, two major SGA in cultivated potato (Solanum tuberosum) are α-chaconine and α-solanine, and their total content in tubers should not exceed the safety limit of 20 mg 100 g~(-1) FW, otherwise it can be toxic to humans. Accumulation of SGAs in potato tubers is influenced by many factors including the key environmental factors, temperature and light. In order to reduce the content of SGAs and research the accumulation mechanism of SGAs in potato tubers, in the present study we attempted to compare the contents of SGAs, second messengers-calmodulin and the G protein in potato tubers, treated with the treatments of red light, blue light, white light and darkness under the same temperature (15 ℃). The results indicated that red light significantly (P < 0.01) increased the average content of SGAs in potato tubers, with 26.02%, 55.50%, and 100.79% higher than that treated with blue light, white light and darkness, respectively. It was confirmed that light with different wavelengths has different effects on accumulation of SGAs in potato tubers. Among factors influencing the accumulation of SGAs in potato tubers, the red light took the first place and the blue light took the second place. Meanwhile, the content of second messengers-the G protein in potato tubers treated with red light was 0.95%, 2.01%, and 3.86% higher than that treated with blue light, white light and darkness, respectively. And the content of calmodulin in potato tubers treated with red light was 7.94%, 37.41%, and 87.24% higher than that treated with blue light, white light and darkness, respectively. It was inferred that the red light is the signal molecule for SGAs accumulation, which stimulates the acceptor (PHYB) of red light and participate in accumulation of SGAs in potato tubers, together with the second messenger G protein, calmodulin, and so on.
