CAJ | 학술논문

本文以昆明小鼠和鸡卵清白蛋白多肽为研究对象，研究了鸡卵清白蛋白多肽对紫外线损伤的小鼠光老化皮肤的保护作用。实验动物随机分为正常对照组、模型对照组、Vc阳性对照组、卵清白蛋白多肽低剂量组、卵清白蛋白多肽高剂量组五组。除正常对照组外，运用紫外灯管模拟紫外线长期照射各组小鼠皮肤的无毛部分，建立小鼠皮肤光老化模型。同时，用5％Vc溶液、10％的鸡卵清白蛋白多肽溶液、40％的鸡卵清白蛋白多肽溶液分别外涂于Vc阳性对照组、卵清白蛋白多肽低剂量组、卵清白蛋白多肽高剂量组小鼠背部无毛皮肤，正常对照组和模型对照组涂抹蒸馏水。60天后，观察各组小鼠皮肤的表观特征并测定相关生化指标。结果显示，与模型对照组相比，鸡卵清白蛋白多肽高、低剂量组小鼠皮肤光滑，褶皱较浅，富有弹性，红斑极少，接近正常对照组小鼠皮肤；皮肤重量均显著降低（p〈0．01，p〈0．01），羟脯氨酸（HPY）含量均显著降低（p〈0．01，p〈0．05），丙二醛（MDA）含量存在显著差异（p〈0．05，p〈0．05），超氧化物歧化酶（SOD）活力显著增强（p〈0．05，p〈0．05）。说明鸡卵清白蛋白多肽对小鼠光老化皮肤有明显的保护作用。
본문이곤명소서화계란청백단백다태위연구대상，연구료계란청백단백다태대자외선손상적소서광노화피부적보호작용。실험동물수궤분위정상대조조、모형대조조、Vc양성대조조、란청백단백다태저제량조、란청백단백다태고제량조오조。제정상대조조외，운용자외등관모의자외선장기조사각조소서피부적무모부분，건립소서피부광노화모형。동시，용5％Vc용액、10％적계란청백단백다태용액、40％적계란청백단백다태용액분별외도우Vc양성대조조、란청백단백다태저제량조、란청백단백다태고제량조소서배부무모피부，정상대조조화모형대조조도말증류수。60천후，관찰각조소서피부적표관특정병측정상관생화지표。결과현시，여모형대조조상비，계란청백단백다태고、저제량조소서피부광활，습추교천，부유탄성，홍반겁소，접근정상대조조소서피부；피부중량균현저강저（p〈0．01，p〈0．01），간포안산（HPY）함량균현저강저（p〈0．01，p〈0．05），병이철（MDA）함량존재현저차이（p〈0．05，p〈0．05），초양화물기화매（SOD）활력현저증강（p〈0．05，p〈0．05）。설명계란청백단백다태대소서광노화피부유명현적보호작용。
In this paper, we presents a through study on the effect of chicken ovalbumin peptide on anti-skin photoaging, using Kunming mouse as material. Kunming mouse was subdivided into five groups, that is： normal control group, model control group, Vc positive control group, low dose group of chicken ovalbumin oeptide, and high dose group of chicken ovalbumin oeptide. Hairless skin on the back of samples in all groups, except for those in the normal control group, were exposed to UV damage to create a skin photoaging model. Meanwhile, hairless skin on the back of animals in Vc positive control group, low dose group of chicken ovalbumin oepfide, and high dose group of chicken ovalbumin oeptide were smeared with 5% Vc, 10% chicken ovalbumin oeptide, and 40% chicken ovalbumin oepfide, respectively. The normal control group and model control group were both smeared with distilled water. Aider 60 days treatment, characteristics and biochemical parameters were measured. The mselt showed that, the skin of animals in low dose group of chicken ovalbumin oepfide and high dose group of chicken ovalbumin oepfide was similar to that of animals in model control group, showing elastic and light wrinkled. Additionaly, both skin weight （p〈0.01, p〈0.01） and hydroxyproline content （p〈0.01, p〈0.05） declinced significantly in those two groups, while malondialdehyde content （p〈0.05, p〈0.05） and superoxide dismutase activity （p〈0.05, p〈0.05） increased. We hence came to the conclusion that chicken ovalbumin oeptide could positively antagonize skin photoaging caused by UV.