CAJ | 학술논문

目的探讨成肌纤维细胞(myofibroblast,MF)在支气管哮喘(简称哮喘)气道重塑中的作用.观察罗红霉素对哮喘气道重塑的影响,并与地塞米松作对照.方法 SD大鼠40只,随机分为哮喘组(A组)、生理盐水对照组(C组)、地塞米松治疗组(D组)和罗红霉素治疗组(R组),每组10只.利用卵白蛋白(ovalbumin,OVA)/Al(OH).致敏与OVA雾化吸人激发建立大鼠哮喘模型.免疫组织化学测定肺组织中支气管上皮下MF的一平滑肌肌动蛋白(α-smooth muscle actin,α-SMA)表达含量,并使用图象分析技术进行积分光密度(integral optical density,IOD)定量分析测定.光镜观察肺组织病理结构变化,图像分析软件分析,并测定肺内支气管总管壁厚度、内管壁厚度、平滑肌层厚度等指标.结果免疫组织化学和图像分析结果:A组与C组相比,总管壁厚度、内管壁厚度、平滑肌层厚度显著增厚(P<0.01).D组和R组中的内管壁厚度、平滑肌层厚度与A组相比.均显著变薄(P<0.01).R组的总管壁厚度、内管壁厚度、平滑肌层厚度与D组相比差异无统计学意义.定量分析测定的IOD值显示A组支气管上皮下MF α-SMA表达含量较C组显著增加(P<0.01),D组和R组表达含量较A组均减少(P<0.05),R组表达含量与D组相比差异无统计学意义.相关分析结果:支气管上皮下MF α-SMA表达含量(用IOD值表示)和内管壁厚度呈正相关(r=0.913,P<0.01,n=40),和平滑肌层厚度也呈正相关(r=0.626.P<0.01,n=40).结论 MF在气道重塑形成中起重要作用.罗红霉索和地塞米松均可能通过抑制MF增殖和表达起到抗哮喘气道重塑作用.
목적 탐토성기섬유세포(myofibroblast,MF)재지기관효천(간칭효천)기도중소중적작용.관찰라홍매소대효천기도중소적영향,병여지새미송작대조.방법 SD대서40지,수궤분위효천조(A조)、생리염수대조조(C조)、지새미송치료조(D조)화라홍매소치료조(R조),매조10지.이용란백단백(ovalbumin,OVA)/Al(OH).치민여OVA무화흡인격발건립대서효천모형.면역조직화학측정폐조직중지기관상피하MF적일평활기기동단백(α-smooth muscle actin,α-SMA)표체함량,병사용도상분석기술진행적분광밀도(integral optical density,IOD)정량분석측정.광경관찰폐조직병리결구변화,도상분석연건분석,병측정폐내지기관총관벽후도、내관벽후도、평활기층후도등지표.결과 면역조직화학화도상분석결과:A조여C조상비,총관벽후도、내관벽후도、평활기층후도현저증후(P<0.01).D조화R조중적내관벽후도、평활기층후도여A조상비.균현저변박(P<0.01).R조적총관벽후도、내관벽후도、평활기층후도여D조상비차이무통계학의의.정량분석측정적IOD치현시A조지기관상피하MF α-SMA표체함량교C조현저증가(P<0.01),D조화R조표체함량교A조균감소(P<0.05),R조표체함량여D조상비차이무통계학의의.상관분석결과:지기관상피하MF α-SMA표체함량(용IOD치표시)화내관벽후도정정상관(r=0.913,P<0.01,n=40),화평활기층후도야정정상관(r=0.626.P<0.01,n=40).결론 MF재기도중소형성중기중요작용.라홍매색화지새미송균가능통과억제MF증식화표체기도항효천기도중소작용.
Objective To explore the effect of myofibroblast (MF) on bronchial asthma(asthma)airway remodeling. To observe the effect of roxithromycin on asthma airway remodeling,and to compare its effect with dexamethasone. Methods In the experiment,40 Sprague-Dawley(SD) rats were randomly divided into the asthma group(group A), the sodium chloride control group (group C), the dexamethasone group (group D) and the roxithromycin group(group R). There were ten rats in each group. The rats were sensitized with ovalbumin and AI(OH)3 before repeatedly exposed to aerosolized ovaibumin. The α-smooth muscle actin (α-SMA) expressions of MF of bronchial subepithelium in lung tissue were assessed by immunohistochemistry. By image analysis technique, their integral optical density(IOD) was quantitatively analyzed. The histopathology and structural change of pulmonary tissues were observed by light microscope.The following important parameters of intrapulmonary bronchus were measured by image analysis technique,too: the total brochial wall thickness,inner airway wall thickness and the smooth muscle thickness. Results The results of immunohistochemistry and image analysis:the total brochial wall thickness,inner airway wall thickness and the smooth muscle thickness in group A were thicker than those in group C(P <0.01). The inner airway wall thickness and the smooth muscle thickness in group D and group R were thinner than those in group A(P <0.01). But there were no significant difference in the total brochial wall thickness, inner airway wall thickness and the smooth muscle thickness between group D and group R. Compared with group C,the IOD value by quantitative analysis shows that expression of MF's α-SMA in bronchial suhepithelium in group A significantly increased(P<0.01). The expression in group D and group R significantly decreased compared with group A (P < 0. 05). But there was no significant difference in their expression between group D and group R. The results of correlation: there were positive correlations between inner airway wall thickness and expression of MF's α-SMA in bronchial subepithelium(presented by IOD)(r =0. 913, P <0.01, n = 40), also positive correlations between the smooth muscle thickness and expression of MF's α-SMA in bronchial subepithelium(presented by IOD)(r = 0. 626, P < 0. 01, n = 40). Conclusions MF plays an important role in the course of airway remodeling. Both roxithromycin and dexamethasone may inhibit proliferation and expression of MF. So they may be effective drugs to inhibit asthma airway remodeling.