CAJ | 학술논문

目的研究葛根素对子痫前期(PE)患者相关Bax、bcl-2及Caspase-3在胎盘绒毛中的表达的影响,探讨葛根素治疗PE对凋亡相关表达的影响及治疗机制.方法选择正常妊娠妇女60例,硫酸镁治疗后PE患者60例(对照组).硫酸镁与葛根素治疗后PE患者54例(研究组),分娩后取胎盘组织,采用免疫组织化学方法检测凋亡相关蛋白的BCL-2,BAX,Caspase-3表达情况;采用末端脱氧核苷酸转移酶介导脱氧核苷酸切口末端标记法(TUNEL法)检测胎盘细胞凋亡情况.结果研究组胎盘绒毛滋养细胞的凋亡率明显高于正常妊娠组,但低于对照组(P<0.05);胎盘绒毛中有Bax、bcl-2、Caspase-3表达主要分布于合体滋养层细胞,研究组Bax和Caspase-3阳性表达率分别为(49.6±5.7)%和(29.5±4.1)%,与正常妊娠比较,表达水平显著增加(P<0.01),与对照组比较,Bax、Caspase-3表达水平显著降低,(P<0.01).结论胎盘组织中Bax、Caspase-3在子痫前期发病中可能起重要作用;葛根素治疗能使胎盘绒毛Bax、Caspase-3表达改变,抑制胎盘绒毛的细胞凋亡,提高子宫-胎盘-胎儿血供.
목적 연구갈근소대자간전기(PE)환자상관Bax、bcl-2급Caspase-3재태반융모중적표체적영향,탐토갈근소치료PE대조망상관표체적영향급치료궤제.방법 선택정상임신부녀60례,류산미치료후PE환자60례(대조조).류산미여갈근소치료후PE환자54례(연구조),분면후취태반조직,채용면역조직화학방법 검측조망상관단백적BCL-2,BAX,Caspase-3표체정황;채용말단탈양핵감산전이매개도탈양핵감산절구말단표기법(TUNEL법)검측태반세포조망정황.결과 연구조태반융모자양세포적조망솔명현고우정상임신조,단저우대조조(P<0.05);태반융모중유Bax、bcl-2、Caspase-3표체주요분포우합체자양층세포,연구조Bax화Caspase-3양성표체솔분별위(49.6±5.7)%화(29.5±4.1)%,여정상임신비교,표체수평현저증가(P<0.01),여대조조비교,Bax、Caspase-3표체수평현저강저,(P<0.01).결론 태반조직중Bax、Caspase-3재자간전기발병중가능기중요작용;갈근소치료능사태반융모Bax、Caspase-3표체개변,억제태반융모적세포조망,제고자궁-태반-태인혈공.
Objective To investigate the expression of apoptosis associated Bax, bcl-2 and Caspase-3 in human placenta of preeclampsia (PE), and to evaluate the influence of Puerarin on apoptosis associated gene and the efficiency mechanism. Methods Placental tissues from 60 normal gravidas, 60 post-treatment women with magnesium sulfate and 54 post-treatmentwomen with magnesium sulfate and Puerarin were collected. TUNEL was used to detect apoptosis rate and immunohistochemical method was used to detect expressions of Bcl-2, Bax and Caspase-3 in the trophocytes of placenta.Results Under the detection of TUNEL technique, it was found that there were more numbers of apoptotic trophoblasts in the placenta of post-treatment with magnesium sulfate and Puerarin than normal gravidas,and fewer apoptotie cells appearing than post-treatment with magnesium sulfate .The expressions of Bax, bcl-2 and Caspase-3 were evident in human placental.dicating positive staining of Bax, and Caspase-3 were respectively49. 6±5.7 and 29.5±4.1 in post-treatment with magnesium sulfate and Puerarin group, which were significantly higher than there ofthe normalgravidas.and were significantly lower than there of the post-treatment with magnesium sulfate groups. Coclusion Bax, and Caspase-3 in placenta may play an important role in the pathogenesis of PE, the Puerarin could reduce the expression of BAX and Caspase-3 of placental, and increase the blood supply of uterus-placenta-fetus and improve therapeutic efficiency.