CAJ | 학술논문

目的了解src抑制的蛋白激酶C底物(SSeCKS)在肝星状细胞(HSC)活化中的变化和作用.方法 (1)分离正常大鼠的HSC,以实时定量PCR检测其在体外培养过程中SSeCKS mRNA的表达变化,以蛋白质印迹法和细胞免疫荧光法检测HSC中SSeCKS蛋白的表达变化.(2)制作肝纤维化的大鼠模型,以免疫荧光法检测肝组织内SSeCKS和α-平滑肌肌动蛋白(α-SMA)的表达变化及定位.结果初分离的HSC表达低水平的SSeCKS mRNA,经培养活化后SSeCKS的表达增强.在纤维化肝组织中,SSeCKS阳性细胞增多,阳性细胞多分布于肝窦周围,与α-SMA阳性细胞的分布一致.结论在体外活化过程中HSC中SSeCKS表达增强.SSeCKS可能参与HSC在肝脏炎症和纤维化中的作用.
목적 료해src억제적단백격매C저물(SSeCKS)재간성상세포(HSC)활화중적변화화작용.방법 (1)분리정상대서적HSC,이실시정량PCR검측기재체외배양과정중SSeCKS mRNA적표체변화,이단백질인적법화세포면역형광법검측HSC중SSeCKS단백적표체변화.(2)제작간섬유화적대서모형,이면역형광법검측간조직내SSeCKS화α-평활기기동단백(α-SMA)적표체변화급정위.결과 초분리적HSC표체저수평적SSeCKS mRNA,경배양활화후SSeCKS적표체증강.재섬유화간조직중,SSeCKS양성세포증다,양성세포다분포우간두주위,여α-SMA양성세포적분포일치.결론 재체외활화과정중HSC중SSeCKS표체증강.SSeCKS가능삼여HSC재간장염증화섬유화중적작용.
Objective To investigate the change and effect of SSeCKS(src suppressed c kinase substrates)in the activation of hepatic stellate cells(HSCs).Methods HSCs were isolated from normal rats,the change of SSeCKS mRNA expression on HSCs culture in vitro was determined using real.time PCR.protein level was determined by Western blot and immunofluorescence methods.A rat model of liver fibrosis was established.The expression and location of SSeCKS and α-SMA(α-smooth muscle actin)in liver tissues were detected by immunofluorescence methods.Results SSeCKS mRNA expression WaS loW in freshly isolated HSCs cell and the expression increased in activated HSCs in vitro.In liver fibrosis tissue,the number of SSeCKS-positive cells was increased and these cells were distributed along the sinusoids which also contained α-SMA positive cells.Conclusion The expression of SSeCKS was increased in activated HSCs in vitro.Therefore.SSeCKS may be involved in the liver inflammation and fibrosis.