CAJ | 학술논문

目的观察低分子肝素(LMWH)对脓毒症大鼠腹主动脉内皮细胞(VEC)蛋白C受体(EPCR)和蛋白酶活化受体1(PAR1)表达的影响.方法采用组织贴块法培养24只Wister大鼠腹主动脉VEC,1:3传代至第4代,分为对照组(n＝6)、脓毒症组(LPS 1 μg/ml,n＝6)、LMWH组(LPS 1 μg/ml+LMWH 5 μg/ml,n＝6).分别在第1、3、5天采用流式细胞术(FCM)检测VEC表面的EPCR和PAR1的表达.结果脓毒症组EPCR和PAR1的表达各时间点均较对照组有明显下降(P＜0.05或P＜0.01),以第5天最为明显(26.53±7.21 vs 39.26±2.62,q＝6.45,P＜0.01;53.21±15.10 vs 86.54±11.34,q＝6.94,P＜0.01).LMWH组EPCR和PAR1的表达各时间点均高于脓毒症组(P＜0.05);与对照组比较,EPCR在第1天仍有明显降低(40.86±1.63 vs 45.41±2.82,q＝3.51,P＜0.05),但第3、5天接近对照组水平(41.20±3.32 vs 42.83±2.66,P＞0.05;39.23±3.33 vs 39.26±2.62,P＞0.05),PAR1各时间点与对照组比较差异均无统计学意义(P＞0.05).结论 LMWH能有效改善脓毒症内皮细胞EPCR和PAR1表达抑制的状态.
목적 관찰저분자간소(LMWH)대농독증대서복주동맥내피세포(VEC)단백C수체(EPCR)화단백매활화수체1(PAR1)표체적영향.방법 채용조직첩괴법배양24지Wister대서복주동맥VEC,1:3전대지제4대,분위대조조(n＝6)、농독증조(LPS 1 μg/ml,n＝6)、LMWH조(LPS 1 μg/ml+LMWH 5 μg/ml,n＝6).분별재제1、3、5천채용류식세포술(FCM)검측VEC표면적EPCR화PAR1적표체.결과 농독증조EPCR화PAR1적표체각시간점균교대조조유명현하강(P＜0.05혹P＜0.01),이제5천최위명현(26.53±7.21 vs 39.26±2.62,q＝6.45,P＜0.01;53.21±15.10 vs 86.54±11.34,q＝6.94,P＜0.01).LMWH조EPCR화PAR1적표체각시간점균고우농독증조(P＜0.05);여대조조비교,EPCR재제1천잉유명현강저(40.86±1.63 vs 45.41±2.82,q＝3.51,P＜0.05),단제3、5천접근대조조수평(41.20±3.32 vs 42.83±2.66,P＞0.05;39.23±3.33 vs 39.26±2.62,P＞0.05),PAR1각시간점여대조조비교차이균무통계학의의(P＞0.05).결론 LMWH능유효개선농독증내피세포EPCR화PAR1표체억제적상태.
Objective To investigate the effect of low molecular weight heparin (LMWH) on the expression of endothelial protein C receptor (EPCR) and protease activated receptor 1 (PAR1) in abdominal vascular endothelial cells (VECs) of septic rats. Methods VECs were cultured by tissue-sticking method, and the purity was determined with flow cytometry (FCM). VECs were randomly divided into three groups: control group, septic group (LPS 1 μg/ml) and LMWH group (LPS 1 μg/ml+LMWH 5 μg/ml). The VECs were collected at 1st, 3rd, 5th days after stimulated. The expression of EPCR and PAR1 were assessed by FCM. Results The expression of EPCR and PAR1 of septic group decreased significantly compared with control group at each time point (P＜0.05 or P＜0.01), and the expression decreased most obviously on day 5 (26.53±7.21 vs 39.26±2.62,q＝6.45,P＜0.01;53.21±15.10 vs 86.54±11.34,q＝6.94,P＜0.01). In LMWH group, the levels of EPCR and PAR1 expression were higher than setpic group at each time point (P＜0.05). Compared to control group, the expression of EPCR had a significantly decrease on day 1 (40.86±1.63 vs 45.41±2.82,q＝3.51,P＜0.05), which had no significantly different on day 3 and 5 (41.20±3.32 vs 42.83±2.66,P＞0.05;39.23±3.33 vs 39.26±2.62,P＞0.05), and the expression of PAR1 were not significantly decrease compared with control group at each time point (P＞0.05). Conclusions LMWH could improve the inhibition status and the expression of EPCR and PAR1 on VECs in septic rats.