CAJ | 학술논문

目的应用根尖诱导的动物模型评价骨形成蛋白(BMP)及MTA对根尖诱导的促进作用.方法利用6月龄Beagle犬的前磨牙制备年轻恒牙根尖停止发育的动物模型,并对不同牙齿根管随机采用四种方法:(1)对照组:开放不充填;(2)Ca(OH)2组:Ca(OH)2充填;(3)MTA组:MTA充填;(4)BMP组:根尖区应用BMP+Ca(OH):糊剂充填.术后3个月处死动物,取标本进行X线检查及常规脱钙切片组织学检查,观察牙根发育及根尖孔封闭状况.结果 X线检查显示,单纯开放的患牙其根尖均未能继续发育;单纯使用Ca(OH)2的患牙根尖继续发育,但根尖封闭不完全;使用MTA及Ca(OH)2+BMP糊剂的牙齿根尖继续发育,根尖封闭完全.组织学检查表明,Ca(OH):组中仅1/6牙齿形成了根尖封闭,MTA组中5/6牙齿根尖形成了根尖的关闭及钙化屏障,BMP组中全部牙齿(6/6)的根尖均形成了根尖的关闭及钙化屏障.结论 6月龄Beagle犬的前磨牙可作为研究根尖诱导成形的动物模型.单纯Ca(OH)2糊剂对根尖诱导成形的作用有限.使用BMP或MTA均可对未发育完全的根尖起到良好的诱导作用,促进根尖的发育及根尖孔的封闭.
목적 응용근첨유도적동물모형평개골형성단백(BMP)급MTA대근첨유도적촉진작용.방법 이용6월령Beagle견적전마아제비년경항아근첨정지발육적동물모형,병대불동아치근관수궤채용사충방법:(1)대조조:개방불충전;(2)Ca(OH)2조:Ca(OH)2충전;(3)MTA조:MTA충전;(4)BMP조:근첨구응용BMP+Ca(OH):호제충전.술후3개월처사동물,취표본진행X선검사급상규탈개절편조직학검사,관찰아근발육급근첨공봉폐상황.결과 X선검사현시,단순개방적환아기근첨균미능계속발육;단순사용Ca(OH)2적환아근첨계속발육,단근첨봉폐불완전;사용MTA급Ca(OH)2+BMP호제적아치근첨계속발육,근첨봉폐완전.조직학검사표명,Ca(OH):조중부1/6아치형성료근첨봉폐,MTA조중5/6아치근첨형성료근첨적관폐급개화병장,BMP조중전부아치(6/6)적근첨균형성료근첨적관폐급개화병장.결론 6월령Beagle견적전마아가작위연구근첨유도성형적동물모형.단순Ca(OH)2호제대근첨유도성형적작용유한.사용BMP혹MTA균가대미발육완전적근첨기도량호적유도작용,촉진근첨적발육급근첨공적봉폐.
Objective To evaluate the efficacy of BMP and MTA for apexification in immature roots of dogs.Methods Six-month-old Beagle dogs'premolars were used to make animal models.Different root canals were adopted 4 methods randomly for 3 months.(1)Exposing the pulp without filling;(2) filling the pulp with Ca(OH)2;(3)filling the pulp with MTA;(4) filled with BMP + Ca(OH)2.The growth of the root and the condition of closure of the apical foramina were evaluated with X-ray and histologic examinations.Results The results showed that the difference in the degree of root growth and amount of closure of the apical foramina among the four groups was significant.The root apexes of the teeth filled with MTA or BMP + Ca(OH)2 could keep growing and the apical foramina were completely closed.In addition,in the MTA group,about 5/6 teeth had apical closure with a calcific barrier while all teeth had apical closure with a calcific barrier in the BMP group.Conclusion Six-month-old Beagle dogs' premolars were suitable for animal models for apexiflcation.The effect of using Ca(OH) 2 simply for apexification is limited.BMP or MTA both could not only have well inducement to the undeveloped root apexes,but also promote the growth and the closure of the apical foramina.It will shorten the treatment of the apexification and increase the success rate.