中华口腔医学杂志
中華口腔醫學雜誌
중화구강의학잡지
Chinese Journal of Stomatology
2009年
7期
413-415
,共3页
赵川江%徐琛蓉%周玉竹%苏小鹏
趙川江%徐琛蓉%週玉竹%囌小鵬
조천강%서침용%주옥죽%소소붕
T淋巴细胞,调节性%流式细胞术%侵袭性牙周炎
T淋巴細胞,調節性%流式細胞術%侵襲性牙週炎
T림파세포,조절성%류식세포술%침습성아주염
T-lymphocytes,regulatory%Flow cytometry%Aggressive periodontitis
目的 探讨侵袭性牙周炎患者外周血中CD4+CD-25+调节性T细胞比例及其免疫抑制功能的变化,以期加深对侵袭性牙周炎免疫调节机制的认识.方法 应用流式细胞仪计数牙周健康者17例、慢性牙周炎患者17例和广泛型侵袭性牙周炎患者16例外周血中CD-4+CD-25+调节性T细胞的比例;免疫磁珠法分离受试者外周血中CD-4+CD-25+调节性T细胞和CD-4+CD-25-T细胞,体外淋巴细胞混合培养法检测CD-4+CD-25+调节性T细胞对同源CD-4+CD-25-T细胞增殖的抑制效应.结果 侵袭性牙周炎患者外周血中CD-4+CD-25+调节性T细胞占CD-4+T细胞的比例为(9.71±4.01)%,显著低于牙周健康者[(14.72±3.51)%]和慢性牙周炎患者[(17.01±5.16)%],差异有统计学意义(P<0.05);当CD-4+CD-25+调节性T细胞与CD-4+CD-25-T细胞的比例为2:1、1:1、1:2时,侵袭性牙周炎患者CD-4+CD-25+调节性T细胞的抑制率[分别为(31.37±5.12)%、(24.47±5.53)%及(19.36±3.58)%]较慢性牙周炎患者[分别为(54.14±8.23)%、(41.72±6.35)%及(28.21±5.31)%]和牙周健康者[分别为(58.02±9.62)%、(45.25 ±8.11)%及(29.56±4.23)%]显著降低(P<0.05).结论 侵袭性牙周炎患者外周血CD-4+CD-25+调节性T细胞在数量上存在异常,并可能伴有抑制功能缺陷.
目的 探討侵襲性牙週炎患者外週血中CD4+CD-25+調節性T細胞比例及其免疫抑製功能的變化,以期加深對侵襲性牙週炎免疫調節機製的認識.方法 應用流式細胞儀計數牙週健康者17例、慢性牙週炎患者17例和廣汎型侵襲性牙週炎患者16例外週血中CD-4+CD-25+調節性T細胞的比例;免疫磁珠法分離受試者外週血中CD-4+CD-25+調節性T細胞和CD-4+CD-25-T細胞,體外淋巴細胞混閤培養法檢測CD-4+CD-25+調節性T細胞對同源CD-4+CD-25-T細胞增殖的抑製效應.結果 侵襲性牙週炎患者外週血中CD-4+CD-25+調節性T細胞佔CD-4+T細胞的比例為(9.71±4.01)%,顯著低于牙週健康者[(14.72±3.51)%]和慢性牙週炎患者[(17.01±5.16)%],差異有統計學意義(P<0.05);噹CD-4+CD-25+調節性T細胞與CD-4+CD-25-T細胞的比例為2:1、1:1、1:2時,侵襲性牙週炎患者CD-4+CD-25+調節性T細胞的抑製率[分彆為(31.37±5.12)%、(24.47±5.53)%及(19.36±3.58)%]較慢性牙週炎患者[分彆為(54.14±8.23)%、(41.72±6.35)%及(28.21±5.31)%]和牙週健康者[分彆為(58.02±9.62)%、(45.25 ±8.11)%及(29.56±4.23)%]顯著降低(P<0.05).結論 侵襲性牙週炎患者外週血CD-4+CD-25+調節性T細胞在數量上存在異常,併可能伴有抑製功能缺陷.
목적 탐토침습성아주염환자외주혈중CD4+CD-25+조절성T세포비례급기면역억제공능적변화,이기가심대침습성아주염면역조절궤제적인식.방법 응용류식세포의계수아주건강자17례、만성아주염환자17례화엄범형침습성아주염환자16예외주혈중CD-4+CD-25+조절성T세포적비례;면역자주법분리수시자외주혈중CD-4+CD-25+조절성T세포화CD-4+CD-25-T세포,체외림파세포혼합배양법검측CD-4+CD-25+조절성T세포대동원CD-4+CD-25-T세포증식적억제효응.결과 침습성아주염환자외주혈중CD-4+CD-25+조절성T세포점CD-4+T세포적비례위(9.71±4.01)%,현저저우아주건강자[(14.72±3.51)%]화만성아주염환자[(17.01±5.16)%],차이유통계학의의(P<0.05);당CD-4+CD-25+조절성T세포여CD-4+CD-25-T세포적비례위2:1、1:1、1:2시,침습성아주염환자CD-4+CD-25+조절성T세포적억제솔[분별위(31.37±5.12)%、(24.47±5.53)%급(19.36±3.58)%]교만성아주염환자[분별위(54.14±8.23)%、(41.72±6.35)%급(28.21±5.31)%]화아주건강자[분별위(58.02±9.62)%、(45.25 ±8.11)%급(29.56±4.23)%]현저강저(P<0.05).결론 침습성아주염환자외주혈CD-4+CD-25+조절성T세포재수량상존재이상,병가능반유억제공능결함.
Objective To investigate the changes of proportion and suppression function of CD-4+ CD-25+ regulatory T cells in the peripheral blood of patients with aggressive periodontitis.Methods Flow cytometric analysis was used to detect the frequency of CD-4+ CD-25+ regulatory T cells in the peripheral blood of 16 patients with generalized aggressive periodontitis and 17 patients with chronic periodontitis,as well as 17 periodontal healthy controls.Furthermore,CD-4+ CD-25+ regulatory T cells and CD-4+ CD-25- T cells were separated from peripheral blood of each enrolling subject using magnetic cell sorting technology.The direct suppression effect of CD-4+ CD-25+ regulatory T cells on CD-4+ CD-25- T lymphocytes proliferation was performed by the mixed lymphocytes reaction and measured by 3H-thymidine radioactive assay.Results The patients with generalized aggressive periodontitis had a lower frequency of CD-4+ CD-25+ regulatory T cells(9.71±4.01)%in the peripheral blood than periodontal healthy controls [(14.72±3.51)%]and chronic periodontitis patients[(17.01±5.16 )%],P＜0.05.A significant decrease was found in the suppression function of CD-4+ CD-25+ regulatory T cells from peripheral blood of patients with generalized aggressive periodontitis when co-cultured with CD-4+ CD-25- T lymphocytes in the proportion of 2:1,1:1 and 1:2 as compared with chronic periodontitis patients and periodontal healthy controls(P＜0.05).Conclusions Diminished numbers and decreased suppression function of CD-4+ CD-25+ regulatory T cells were found in patients with generalized aggressive periodontitis.
