CAJ | 학술논문

目的应用基因表达谱芯片筛查胃癌相关差异表达基因.方法采用含有14784条人类全长基因的cDNA表达谱芯片,从7例胃癌及对应正常胃黏膜组织标本中,筛选胃癌相关差异表达基因,应用逆转录-聚合酶链反应(RT-PCR)验证部分差异表达基因.结果 7例胃癌组织中共检出35个差异表达基因,18个基因表达呈显著上调,17条基因表达呈显著下调.选择7个差异表达明显基因在7例胃癌组织中进行RT-PCR验证,其中5个基因表达上调(S100A6、S100A11、ETV4、CDH17和EphB4)、2个基因表达下调(NK4和PPP2R1B),经RT-PCR验证其表达趋势与基因芯片检测结果一致.结论胃癌组织与正常胃黏膜组织间存在明显差异基因,部分差异表达基因经RT-PCR验证,S100A6、S100A11、ETV4、CDH17、NK4、PPP2R1B和Ephb4基因异常表达可能参与胃癌发生发展.
목적 응용기인표체보심편사사위암상관차이표체기인.방법 채용함유14784조인류전장기인적cDNA표체보심편,종7례위암급대응정상위점막조직표본중,사선위암상관차이표체기인,응용역전록-취합매련반응(RT-PCR)험증부분차이표체기인.결과 7례위암조직중공검출35개차이표체기인,18개기인표체정현저상조,17조기인표체정현저하조.선택7개차이표체명현기인재7례위암조직중진행RT-PCR험증,기중5개기인표체상조(S100A6、S100A11、ETV4、CDH17화EphB4)、2개기인표체하조(NK4화PPP2R1B),경RT-PCR험증기표체추세여기인심편검측결과일치.결론 위암조직여정상위점막조직간존재명현차이기인,부분차이표체기인경RT-PCR험증,S100A6、S100A11、ETV4、CDH17、NK4、PPP2R1B화Ephb4기인이상표체가능삼여위암발생발전.
Objective To screen genes differentially expressed in clinical samples of resected gastric carcinoma and their adjacent normal gastric tissues by gene expression profile chip.Methods Microarrays containing 14 784 individual full length cDNAs from homo sapiens were applied to screen genes differentially expressed in 7 clinical samples of resected gastric carcinoma and their adjacent normal gastric tissues.Furthermore,we have confirmed differentially expressed genes in gastric carcinoma tissue by reverse transcription-polymerase chain reaction (RT-PCR).Results Compared with normal gastric mucosae,35 differentially expressed genes were detected in 7 cases of gastric carcinoma tissue,including 18 genes with up-regulated expression and 17 genes with down-regulated expression.In order to verify the differentially expressed genes detected from cDNA chip experiment,7 genes with most altered expression were chosen from 7 cases of gastric carcinoma to be verified by RT-PCR,of which 5 genes were confirmed to be up-regulated (S100A6,S100A11,ETV4,CDH17 and EphB4) and 2 genes to be down-regulated (NK4 and PPP2R1B).Above all genes in gastric carcinoma were verified by RT-PCR to be in accordance with the pattern of gene microarray detection.Conclusion Microarray analysis is efficient in the screening of differentially expressed genes related to gastric carcinoma.As proved by RT-PCR on partial genes that are differentially expressed,the altered expression of S100A6,S100A11,ETV4,CDHI7,NK4,PPP2R1B and EphB4 might be involved in the oncogenesis and progression of gastric carcinoma.