CAJ | 학술논문

目的探讨补体C4b及甲状腺素转运蛋白(TTR)在增生性玻璃体视网膜病变(PVR)中的表达变化及意义.方法对照实验研究.提取5例PVR患者玻璃体标本,以10例眼球捐献者的玻璃体标本作为正常对照组,进行双向电泳分析.应用Image master软件分析凝胶图谱获得的差异性蛋白质点,再经质谱分析技术鉴定出差异性蛋白质.兔眼玻璃体腔内注射视网膜色素上皮细胞,制造兔PVR模型,采用酶联免疫吸附测定(ELISA)法检测玻璃体中差异性蛋白质的浓度,以进一步验证PVR患者的蛋白质组学结果.PVR患者组与正常对照组玻璃体中差异性蛋白质浓度比较采用配对样本t检验.结果双向电泳凝胶图谱显示PVR患者组与正常对照组玻璃体中有79个差异表达蛋白质点,对其中9个表达上调的差异性蛋白质点进行质谱鉴定,分别为补体C4b、TTR及7个血清蛋白.经ELISA法检测,正常对照组玻璃体中补体C4b和TTR浓度分别为(20.18±1.97)mg/L和(88.58±8.84)mg/L,PVR患者组玻璃体中补体和TTR浓度分别为(38.1±5.79)mg/L和(112.57±6.89)mg/L;PVR患者组玻璃体中的补体C4b和TTR浓度明显高于正常对照组,差异有统计学意义(C4b:t=11.54,TTR:t=9.24;P＜0.05).结论 PVR患者组与正常对照组玻璃体中补体C4b和TTR表达存在差异.补体C4b和TTR的表达上调可能与PVR的发病机制有关.
목적 탐토보체C4b급갑상선소전운단백(TTR)재증생성파리체시망막병변(PVR)중적표체변화급의의.방법 대조실험연구.제취5례PVR환자파리체표본,이10례안구연헌자적파리체표본작위정상대조조,진행쌍향전영분석.응용Image master연건분석응효도보획득적차이성단백질점,재경질보분석기술감정출차이성단백질.토안파리체강내주사시망막색소상피세포,제조토PVR모형,채용매련면역흡부측정(ELISA)법검측파리체중차이성단백질적농도,이진일보험증PVR환자적단백질조학결과.PVR환자조여정상대조조파리체중차이성단백질농도비교채용배대양본t검험.결과 쌍향전영응효도보현시PVR환자조여정상대조조파리체중유79개차이표체단백질점,대기중9개표체상조적차이성단백질점진행질보감정,분별위보체C4b、TTR급7개혈청단백.경ELISA법검측,정상대조조파리체중보체C4b화TTR농도분별위(20.18±1.97)mg/L화(88.58±8.84)mg/L,PVR환자조파리체중보체화TTR농도분별위(38.1±5.79)mg/L화(112.57±6.89)mg/L;PVR환자조파리체중적보체C4b화TTR농도명현고우정상대조조,차이유통계학의의(C4b:t=11.54,TTR:t=9.24;P＜0.05).결론 PVR환자조여정상대조조파리체중보체C4b화TTR표체존재차이.보체C4b화TTR적표체상조가능여PVR적발병궤제유관.
Objective To investigate the differential expression of complement C4b and transthyretin in proliferative vitreoretinopathy (PVR). Methods It was a controlled experimental study.Human vitreous samples of 5 patients with PVR were analyzed by using two-dimensional gel electrophoresis and mass spectrometry,and the results were compared with those from normal control vitreous obtained from donor eyes. An in vivo model of PVR was created by intravitreous injection of cultured rabbit retinal pigment epithelial (RPE) cells. The vitreous of PVR models were analyzed by enzyme linked immunosorbent assay (ELISA) to confirm the proteomic results from the PVR patients. Results Seventy nine various proteins were expressed differently between PVR and normal vitreous,among which nine up-regulated proteins including complement C4b,transthyretin (TTR),and 7 albumins were identified by mass spectrometry. The up-regulation of complement C4b and TTR in PVR patients was also confirmed by ELISA. The concentration of complement C4b and TTR in normal vitreous were(20. 18 ± 1.97)mg/L and (88.58 ± 8.84)mg/L respectively,in PVR patients were ( 38.1 ± 5.79) mg/L and ( 112.57 ± 6.. 89 ) mg/L repectively,difference significantly between these two groups ( C4b: t = 11. 54,TTR: t = 9. 24;P ＜ 0. 05 ). Conclusions Differences of complement C4b and TTR expression were observed between PVR and normal vitreous. These results have lead to the assumption that there is a connection between elevated concentrations of both complement C4b and TTR and the pathogenesis of PVR and further studies on the functions of these proteins are required.