CAJ | 학술논문

目的探讨视网膜母细胞瘤(RB)组织中热休克蛋白(HSP)70、90的表达及其与凋亡调控因子Survivin表达关系,以及两者共同表达对RB细胞增生活性的影响. 方法采用链霉素抗生物素蛋白-过氧化物酶免疫组织化学法检测43例RB及6例正常视网膜石蜡组织切片HSP70、HSP90、Survivin和Ki-67蛋白表达水平,以Ki-67蛋白标记指数作为肿瘤细胞增生活性标志,结合病理学特征分析HSPs-Survivin协同表达与肿瘤细胞增生活性关系. 结果 43例RB组织中HSP70阳性表达28例,占65.12%,HSP90阳性表达37例,占86.05%,Survivin阳性表达27例,占62.79%;6例正常视网膜组织中均无HSP70、HSP90及Survivin表达;HSP90阳性表达组中Survivin阳性表达率明显高于阴性表达组(P<0.05);HSP90阳性表达组及Survivin阳性表达组Ki-67蛋白标记指数均较表达阴性组明显增高(P<0.05),HSP90-Survivin共同阳性表达组Ki-67蛋白标记指数较HSP90-Survivin共同表达阴性或表达不一致组明显增高(P<0.05).但HSP70表达与Survivin相关性无统计学意义(P>0.05),且对Ki-67蛋白标记指数无明显影响(P>0.05);HSPs、Survivin表达及Ki-67蛋白标记指数与RB组织学分型无关(P均>0.05).结论 RB组织中HSP90与Survivin表达存在明显相关性,HSP90-Survivin共同阳性表达可能在RB细胞增生机制中具有重要意义.
목적 탐토시망막모세포류(RB)조직중열휴극단백(HSP)70、90적표체급기여조망조공인자Survivin표체관계,이급량자공동표체대RB세포증생활성적영향. 방법채용련매소항생물소단백-과양화물매면역조직화학법검측43례RB급6례정상시망막석사조직절편HSP70、HSP90、Survivin화Ki-67단백표체수평,이Ki-67단백표기지수작위종류세포증생활성표지,결합병이학특정분석HSPs-Survivin협동표체여종류세포증생활성관계. 결과 43례RB조직중HSP70양성표체28례,점65.12%,HSP90양성표체37례,점86.05%,Survivin양성표체27례,점62.79%;6례정상시망막조직중균무HSP70、HSP90급Survivin표체;HSP90양성표체조중Survivin양성표체솔명현고우음성표체조(P<0.05);HSP90양성표체조급Survivin양성표체조Ki-67단백표기지수균교표체음성조명현증고(P<0.05),HSP90-Survivin공동양성표체조Ki-67단백표기지수교HSP90-Survivin공동표체음성혹표체불일치조명현증고(P<0.05).단HSP70표체여Survivin상관성무통계학의의(P>0.05),차대Ki-67단백표기지수무명현영향(P>0.05);HSPs、Survivin표체급Ki-67단백표기지수여RB조직학분형무관(P균>0.05).결론 RB조직중HSP90여Survivin표체존재명현상관성,HSP90-Survivin공동양성표체가능재RB세포증생궤제중구유중요의의.
Objective To investigate the relationship of the expression between heat shock protein (HSP) 70 and 90, and Survivin and its effects on the proliferative activity in retinoblastoma (RB) cells. Methods Expression of Survivin, HSP70 and 90, and Ki-67 in conventional paraffin samples from 43 patients with RB and 6 healthy people was detected by streptavidin-biotin peroxidase (SP) immunohistochemical method. Ki-67 labeling index was used to evaluate the proliferative activity in RB. Results In 43 cases of RB, positive expression of HSP70 and 90 and Survivin was found in 28 (65.12%), 37 (86.05%) and 27 (62.79%) cases, respectively. None of the 6 normal retinal tissue expressed HSP70, HSP90 or Survivin. Positive expression of Survivin was more frequent in positive expressions of HSP90 than that in negative expressions of HSP90 (P<0.05). Ki-67 labeling index was higher in positive expressions of HSP90 and positive expressions of Survivin than that in their negative expressions respectively (P<0.05). Meanwhile, higher Ki-67 labeling index was found in positive HSP90-Survivin expressions than that in negative HSP90-Survivin expressions and those cases where only HsP90 or Survivin was found (P<0.05). Expression of HSP70 did not correlate with that of Survivin, nor had any significant effect on Ki-67 labeling index (P>0.05). Expression of HSPs and Survivin and Ki-67 labeling index did not correlate with histological types (P>0.05). Conclusion Expression of HSP90 correlates with that of Survivin in RB. Co-existence of Survivin and HSP90 probably plays an important role in the genesis of RB.