CAJ | 학술논문

目的：研究血清IL-35和外周血Treg细胞含量在慢性乙型肝炎(HBV)患者中的作用及意义。方法选取广东医学院附属厚街医院感染科慢性HBV感染患者40例[(其中慢性乙肝无症状携带者(AsC)8例，慢性乙肝轻、中度患者(CHB-LM)20例和慢性乙肝重型患者(CSHB)12例]作为实验组。同时选取本院体检科健康志愿者(HV)15例作为对照组。采用ELISA方法检测血清IL-35含量，流式细胞术检测外周血Treg细胞含量， Pearson相关分析两者的相关性。结果 HBV患者血清IL-35含量[(75.00±6.75) pg/ml]显著高于健康志愿者血清IL-35含量[(44.13±4.49) pg/ml]，两者比较差异有统计学意义(P<0.05)。CSHB组和CHB-LM组血清IL-35含量均明显高于AsC组(P<0.05)；CSHB组和CHB-LM组间血清IL-35含量差异无统计学意义(P>0.05)。HBV患者外周血Treg细胞含量[(6.45±0.42)%]显著高于健康志愿者外周血Treg细胞含量[(3.07±0.31)%]，两者比较差异有统计学意义(P<0.05)。CSHB组Treg细胞含量明显高于CHB-LM组和AsC组Treg细胞含量(P均<0.05)。CHB-LM组Treg细胞含量明显高于AsC组Treg细胞含量(P<0.05)。HBV患者血清IL-35含量与外周血Treg细胞含量呈正相关(P<0.05)。结论血清IL-35和外周血Treg细胞含量在慢性乙型病毒性肝炎不同疾病阶段存在差异，可能在HBV发生、发展过程中发挥重要作用。
목적：연구혈청IL-35화외주혈Treg세포함량재만성을형간염(HBV)환자중적작용급의의。방법선취엄동의학원부속후가의원감염과만성HBV감염환자40례[(기중만성을간무증상휴대자(AsC)8례，만성을간경、중도환자(CHB-LM)20례화만성을간중형환자(CSHB)12례]작위실험조。동시선취본원체검과건강지원자(HV)15례작위대조조。채용ELISA방법검측혈청IL-35함량，류식세포술검측외주혈Treg세포함량， Pearson상관분석량자적상관성。결과 HBV환자혈청IL-35함량[(75.00±6.75) pg/ml]현저고우건강지원자혈청IL-35함량[(44.13±4.49) pg/ml]，량자비교차이유통계학의의(P<0.05)。CSHB조화CHB-LM조혈청IL-35함량균명현고우AsC조(P<0.05)；CSHB조화CHB-LM조간혈청IL-35함량차이무통계학의의(P>0.05)。HBV환자외주혈Treg세포함량[(6.45±0.42)%]현저고우건강지원자외주혈Treg세포함량[(3.07±0.31)%]，량자비교차이유통계학의의(P<0.05)。CSHB조Treg세포함량명현고우CHB-LM조화AsC조Treg세포함량(P균<0.05)。CHB-LM조Treg세포함량명현고우AsC조Treg세포함량(P<0.05)。HBV환자혈청IL-35함량여외주혈Treg세포함량정정상관(P<0.05)。결론혈청IL-35화외주혈Treg세포함량재만성을형병독성간염불동질병계단존재차이，가능재HBV발생、발전과정중발휘중요작용。
Objective To explore the changes and clinical significances of serum IL-35 and Treg cell in pa-tients with chronic hepatitis B. Methods Fourty cases in the Department of Infectious Diseases of Houjie Hospital of Guangdong Medical College with HBV patients were categrized into three group:Group AsC(8 patients with asymp-tomatic HBV carriers), Group CHB-LM (20 patients with chronic hepatitis B with low and moderate), and Group CSHB (12 patients with chronic severe hepatitis B). The Group HV included 15 healthy volunteers. The serum of IL-35 were measured with Enzyme-linked immunosorbent assay(ELISA),Treg cell concentration in peripheral blood were determined by Flow cytometry. Pearson correlation analysis was used to analyze the correlation of two indepen-dent samples. Results HBV patients had a significantly higher the level of IL-35 [(75.00±6.75) pg/ml] than healthy volunteers [(44.13 ± 4.49) pg/ml]. Significant difference was found between two groups (P<0.05). The levels of IL-35 both on CSHB and CHB-LM group were significantly higher than AsC group (P<0.05), but the levels of IL-35 be-tween CSHB and CHB-LM group were no significantly difference (P>0.05). The Treg cell concentration in the periph-eral blood of HBV patients [(6.4 ±0.42)%] was significantly higher than healthy volunteers [(3.07±0.31)%], the differ-ence of them were statistically significant (P<0.05). The Treg cell concentration on CSHB group was significantly higher than CHB-LM or AsC group (P<0.05). The level of serum IL-35 on HBV patients was positively correlated with the concent of Treg cell in peripheral blood (P<0.05). Conclusion The changes of serum IL-35 and Treg cell concentration were difference during different disease phases in chronic hepatitis B, they may play a more important role in the occurrence and worsening process of chronic HBV infection.