CAJ | 학술논문

　　以暗培养3 d的烟草叶片为受体，以Thaumatin为目的基因，应用根癌农杆菌介导法对烟草进行遗传转化，同时以烟草叶片为材料，对影响转化的几个因素进行优化研究，结果表明：侵染20 min、美罗培南浓度为25 mg/L时有利于提高转化率；经浓度为2．0 mg/L的PPT筛选获得的抗性植株经PCR、GUS组织化学染色和Southern杂交分析鉴定，初步证明外源基因Thaumatin已整合到烟草的基因组中。
　　이암배양3 d적연초협편위수체，이Thaumatin위목적기인，응용근암농간균개도법대연초진행유전전화，동시이연초협편위재료，대영향전화적궤개인소진행우화연구，결과표명：침염20 min、미라배남농도위25 mg/L시유리우제고전화솔；경농도위2．0 mg/L적PPT사선획득적항성식주경PCR、GUS조직화학염색화Southern잡교분석감정，초보증명외원기인Thaumatin이정합도연초적기인조중。
Tobacco leaves under dark culture for 3 days were used as receptor, and the objective gene Thaumatin was trans-formed by Agrobactorium tumefaciens mediated genetic transformation.The transformation efficiency was affected by several factors , which were optimized.The results showed that infection time of 20 min and Meropenem of 25 mg/L were beneficial to the increase in the transformation efficiency.PPT of 2.0 mg/L was used to screen resistant plant , and the resistant plant was identified by PCR , GUS histochemical staining and Southern blot analysis .These confirmed that the exogenous gene Thaumatin had been integrated into the genome of tobacco.