江西农业学报
江西農業學報
강서농업학보
ACTA AGRICULTURAE JIANGXI
2013年
7期
34-36
,共3页
彭玉辅%管帮富%俞燕芳%彭火辉%彭华%陈华玲
彭玉輔%管幫富%俞燕芳%彭火輝%彭華%陳華玲
팽옥보%관방부%유연방%팽화휘%팽화%진화령
长柄紫果槭%愈伤组织%外植体%组织培养
長柄紫果槭%愈傷組織%外植體%組織培養
장병자과축%유상조직%외식체%조직배양
Acer cordatum var.subtrinervium%Callus%Explant%Tissue culture
对长柄紫果槭的嫩茎、叶柄和叶片进行愈伤组织的诱导研究,结果表明各个外植体诱导愈伤组织的难易程度为嫩茎>叶柄>叶片;6月份采摘的外植体分化能力强,褐化少,染菌率低,最适合诱导愈伤组织;MS+6-BA1.0mg/L+NAA0.3mg/L组合诱导愈伤组织诱导率为63%,诱导出的愈伤组织结构致密;MS+6-BA1.0mg/L+NAA0.2mg/L+KT1.0mg/L组合的继代培养基,增殖系数达到2.9。
對長柄紫果槭的嫩莖、葉柄和葉片進行愈傷組織的誘導研究,結果錶明各箇外植體誘導愈傷組織的難易程度為嫩莖>葉柄>葉片;6月份採摘的外植體分化能力彊,褐化少,染菌率低,最適閤誘導愈傷組織;MS+6-BA1.0mg/L+NAA0.3mg/L組閤誘導愈傷組織誘導率為63%,誘導齣的愈傷組織結構緻密;MS+6-BA1.0mg/L+NAA0.2mg/L+KT1.0mg/L組閤的繼代培養基,增殖繫數達到2.9。
대장병자과축적눈경、협병화협편진행유상조직적유도연구,결과표명각개외식체유도유상조직적난역정도위눈경>협병>협편;6월빈채적적외식체분화능력강,갈화소,염균솔저,최괄합유도유상조직;MS+6-BA1.0mg/L+NAA0.3mg/L조합유도유상조직유도솔위63%,유도출적유상조직결구치밀;MS+6-BA1.0mg/L+NAA0.2mg/L+KT1.0mg/L조합적계대배양기,증식계수체도2.9。
In this experiment, various explants (tender stem, leafstalk and leaf) derived from Acer cordatum var.subtrinervium were used to induce callus.The results showed that the tender stem was the best (easiest) explant for the inducement of callus , fol-lowed by leafstalk and leaf.The explants picked in June had strong differentiation ability , less browning and low polluted rate, and were most suitable for the callus inducement .The inducement rate of callus in MS +6-BA 1.0 mg/L+NAA 0.3 mg/L was 63%, and the induced callus had dense structure .In the subculture medium combination MS +6-BA 1.0 mg/L+NAA 0.2 mg/L+KT 1.0 mg/L, the proliferation coefficient reached 2.9.