CAJ | 학술논문

　　目的观察母代高脂饮食和后天高脂饮食对子代大鼠代谢状态的不同影响，以探索母代高脂饮食与子代大鼠成年后代谢性疾病发生的关系。方法将成年SD大鼠分成对照组（C组）和高脂饮食组（H组），至10周龄时交配怀孕生育子代大鼠，子代大鼠断乳后每组再分成高脂饮食组（HH组，CH组）和普通饮食组（HC组，CC组），分别至子代大鼠3周龄和8周龄时观察其代谢相关性指标以及肝脏病理表现。结果H组母鼠孕前体质量、糖耐量曲线下面积、总胆固醇及三酰甘油均明显高于C组母鼠（P<0.05）；3周龄时H组母鼠所生育并喂养的子代大鼠其体质量明显高于C组母鼠生育的子代大鼠（P=0.002），但两者的糖耐量曲线下面积差异无统计学意义（P＞0.05）；至8周龄时各组子代大鼠空腹血糖和胰岛素水平差异无统计学意义，而母鼠为H组的子代大鼠体质量和糖耐量曲线下面积大于母鼠为C组子代大鼠（母代饮食主效应P分别为0.024和0.013），HH组和CH组子代大鼠糖耐量曲线下面积亦大于HC组和CC组的子代大鼠（子代饮食主效应P=0.041）；8周龄时HH组和CH组子代大鼠血清总胆固醇、三酰甘油和低密度脂蛋白水平均高于HC组和CC组（子代饮食主效应P分别为0.008，0.007和0.000）；8周龄时HH组、HC组和CH组子代大鼠表现出不同程度的脂肪肝；CC组子代大鼠肝脏镜下结构均显示正常。结论母代高脂饮食可以引起子代大鼠成年后体质量增加，糖耐量减退以及肝脏脂肪变，增加子代发生代谢性疾病的危险性。
　　목적관찰모대고지음식화후천고지음식대자대대서대사상태적불동영향，이탐색모대고지음식여자대대서성년후대사성질병발생적관계。방법장성년SD대서분성대조조（C조）화고지음식조（H조），지10주령시교배부잉생육자대대서，자대대서단유후매조재분성고지음식조（HH조，CH조）화보통음식조（HC조，CC조），분별지자대대서3주령화8주령시관찰기대사상관성지표이급간장병리표현。결과H조모서잉전체질량、당내량곡선하면적、총담고순급삼선감유균명현고우C조모서（P<0.05）；3주령시H조모서소생육병위양적자대대서기체질량명현고우C조모서생육적자대대서（P=0.002），단량자적당내량곡선하면적차이무통계학의의（P＞0.05）；지8주령시각조자대대서공복혈당화이도소수평차이무통계학의의，이모서위H조적자대대서체질량화당내량곡선하면적대우모서위C조자대대서（모대음식주효응P분별위0.024화0.013），HH조화CH조자대대서당내량곡선하면적역대우HC조화CC조적자대대서（자대음식주효응P=0.041）；8주령시HH조화CH조자대대서혈청총담고순、삼선감유화저밀도지단백수평균고우HC조화CC조（자대음식주효응P분별위0.008，0.007화0.000）；8주령시HH조、HC조화CH조자대대서표현출불동정도적지방간；CC조자대대서간장경하결구균현시정상。결론모대고지음식가이인기자대대서성년후체질량증가，당내량감퇴이급간장지방변，증가자대발생대사성질병적위험성。
Objectives To examine the association of the maternal high-fat (HF) diet with increased susceptibility to obe-sity and the development of metabolic diseases in their offspring, and observe difference in the effect of maternal vs. acquired high fat diet on metabolic state in their offspring. Methods A total of 15 SD female rats were divided into HF diet group (group H, n=9) and control diet group (group C, n=6). After fed on different diet for seven weeks, they were mated at the age of ten weeks and became pregnant. Their offspring were then divided to groups CH and HH fed HF diet and groups CC and HC fed control diet. At the age of 3 and 8 weeks, the metabolic markers and the liver pathohistological evidences of their offspring were obtained. Results The body weight, area under curve (AUC) of glucose tolerance, cholesterol and triglyceride were all higher in group H than those in group C (P<0.05) before pregnancy. The offspring of group H had a higher body weight than the offspring of group C at the age of 3 weeks (P=0.002), and no difference in AUC was found between two groups (P>0.05). At the age of 8 weeks, there was no difference in fasting glucose and insulin levels among the four offspring groups. The AUC and body weight were higher in group H than in group C (main effect of maternal diet, P=0.024, P=0.013). The AUCs were also higher in groups CH and HH than groups CC and HC respectively (main effect of acquired diet, P=0.041). The levels of total cholesterol, triglycerides and LDL at the age of 8 weeks were all higher in HH and CH groups than those in HC and CC groups (main effect of acquired diet, P=0.008, 0.007, 0.000, respectively). Their histological analysis at 8 weeks showed different degrees of fatty liver in HH, HC and CH groups, and normal liver in CC group. Conclusions Maternal HF diet may result in increased body weight, fatty liver and impaired glucose tolerance in their adult offspring, and thus increase the risk of developing metabolic diseases at their later age. .