临床儿科杂志
臨床兒科雜誌
림상인과잡지
2013年
7期
632-636
,共5页
丁宇%缪艳%王翔%汤燕静%薛惠良%陈静%丁丽霞%汤静燕%李本尚%沈树红
丁宇%繆豔%王翔%湯燕靜%薛惠良%陳靜%丁麗霞%湯靜燕%李本尚%瀋樹紅
정우%무염%왕상%탕연정%설혜량%진정%정려하%탕정연%리본상%침수홍
白介素15%急性淋巴细胞白血病%单核苷酸多态性%儿童
白介素15%急性淋巴細胞白血病%單覈苷痠多態性%兒童
백개소15%급성림파세포백혈병%단핵감산다태성%인동
interleukin-15%acute lymphoblastic leukemia%single nucleotide polymorphisms%child
目的探讨白介素15（IL-15）单核苷酸多态性（SNP）位点与中国儿童急性淋巴细胞白血病（ALL）治疗反应的相关性。方法提取ALL患儿缓解期的骨髓细胞基因组DNA，采用质谱阵列技术（MassArray）分析IL-15的5个SNP位点（rs10519612、rs10519613、rs17007695、rs17015014、rs35964658），并对各SNP位点与诱导治疗结束后微小残留病（MRD）进行相关性分析。结果SNP rs17007695与儿童ALL早期诱导治疗结束后的MRD相关（P=0.049），CC基因型诱导结束时MRD阳性率是TT基因型的1.8倍；5个SNPs的单倍型分析显示，CACGG在MRD阳性组中出现的频率高于MRD阴性组，差异有统计学意义（10.5%对4.9%，P=0.035）。结论IL-15相关的SNP与儿童ALL早期治疗反应相关。
目的探討白介素15（IL-15）單覈苷痠多態性（SNP）位點與中國兒童急性淋巴細胞白血病（ALL）治療反應的相關性。方法提取ALL患兒緩解期的骨髓細胞基因組DNA，採用質譜陣列技術（MassArray）分析IL-15的5箇SNP位點（rs10519612、rs10519613、rs17007695、rs17015014、rs35964658），併對各SNP位點與誘導治療結束後微小殘留病（MRD）進行相關性分析。結果SNP rs17007695與兒童ALL早期誘導治療結束後的MRD相關（P=0.049），CC基因型誘導結束時MRD暘性率是TT基因型的1.8倍；5箇SNPs的單倍型分析顯示，CACGG在MRD暘性組中齣現的頻率高于MRD陰性組，差異有統計學意義（10.5%對4.9%，P=0.035）。結論IL-15相關的SNP與兒童ALL早期治療反應相關。
목적탐토백개소15（IL-15）단핵감산다태성（SNP）위점여중국인동급성림파세포백혈병（ALL）치료반응적상관성。방법제취ALL환인완해기적골수세포기인조DNA，채용질보진렬기술（MassArray）분석IL-15적5개SNP위점（rs10519612、rs10519613、rs17007695、rs17015014、rs35964658），병대각SNP위점여유도치료결속후미소잔류병（MRD）진행상관성분석。결과SNP rs17007695여인동ALL조기유도치료결속후적MRD상관（P=0.049），CC기인형유도결속시MRD양성솔시TT기인형적1.8배；5개SNPs적단배형분석현시，CACGG재MRD양성조중출현적빈솔고우MRD음성조，차이유통계학의의（10.5%대4.9%，P=0.035）。결론IL-15상관적SNP여인동ALL조기치료반응상관。
Objectives To investigate the correlation between single nucleotide polymorphisms (SNP) in interleu-kin-15 (IL-15) and treatment response in childhood acute lymphoblastic leukemia (ALL). Methods Genomic DNA samples extracted from remission bone marrow cells of ALL patients were genotyped by MassArray. Five SNPs (rs10519612, rs10519613, rs17007695, rs17015014 and rs35964658) in IL-15 and their association to minimal residual disease (MRD) status in the end of induction therapy were studied. Results SNP rs17007695 was associated with the early response in children with ALL(P=0.049) and the incidence of positive MRD after induction therapy in CC genotype carriers was 1.8 times more than that in TT genotype carriers. Haplotype analysis of these five SNPs showed that the frequency of haplotype CACGG in MRD positive group was 2.1 times higher than that in MRD negative group (P=0.035). Conclusions IL-15 gene polymorphism was associated with the early treatment response in Han Chinese children with acute lymphoblastic leuke-mia.
