CAJ | 학술논문

目的：观察黄芪对慢性哮喘豚鼠模型气道重塑的影响及机制。方法 SPF级雄性豚鼠40只随机分成：对照组、模型组、黄芪注射液组、地塞米松组，每组10只。采取HE染色观察气道重塑，图像分析仪分析肺组织气道重塑情况，免疫组化方法测气道中TGF-β1蛋白的表达。结果与对照组相比，模型组气道壁结构发生改变，支气管管腔变窄，气道上皮细胞脱落，平滑肌增生/增殖肥大。经黄芪或地塞米松治疗后上述改变明显减轻。模型组支气管壁厚度、支气管壁平滑肌厚度、支气管壁平滑肌细胞核数量分别较对照组显著增加。模型组TGF-β1蛋白的积分光密度值( IOD)均较正常组明显增高，黄芪治疗后TGF-β1 IOD值显著下降。结论黄芪注射液能够抑制慢性哮喘模型的气道重塑，其机制可能通过抑制TGF-β1的表达有关。
목적：관찰황기대만성효천돈서모형기도중소적영향급궤제。방법 SPF급웅성돈서40지수궤분성：대조조、모형조、황기주사액조、지새미송조，매조10지。채취HE염색관찰기도중소，도상분석의분석폐조직기도중소정황，면역조화방법측기도중TGF-β1단백적표체。결과여대조조상비，모형조기도벽결구발생개변，지기관관강변착，기도상피세포탈락，평활기증생/증식비대。경황기혹지새미송치료후상술개변명현감경。모형조지기관벽후도、지기관벽평활기후도、지기관벽평활기세포핵수량분별교대조조현저증가。모형조TGF-β1단백적적분광밀도치( IOD)균교정상조명현증고，황기치료후TGF-β1 IOD치현저하강。결론황기주사액능구억제만성효천모형적기도중소，기궤제가능통과억제TGF-β1적표체유관。
Objective To investigate the expression of TGF-β1 in the airway epithelial cell of asthmatic guinea pigs and study the effects of Huang Qi on airway remolding in asthma. Methods Forty adult male guinea pigs were randomly divided into control,asthmatic,arsenic Huang Qi and dexamethasone groups. HE staining and the image analysis were used to analyse the information of the airway remodeling. Immunohistochemistry was used to detect the expression of TGF-β1 in the lung tissue. Results Compared to control group,the model group showed that proliferation of airway smooth muscle,recon-struction of air passage,narrowing of bronchial lumen and swelling of airway epithelial cells were significantly milder after treatment with Huang Qi or dexamethasone. The airway wall and smooth muscle layer were significantly thicker in asthma group than that of control group,the number of smooth muscle cell nucleus increased significantly. The integrated optical den-sity ( IOD) value of TGF-β1 were statistically significantly higher than that in control which was derreased after treated with Huang Qi. Conclusions There was TGF-β1-overproduction in airway epithelial cells of asthmatic guinea-pigs,which may contribute to airway remolding in asthma. Astragus could decrease airway remolding by inhibiting the expression of TGF-β1.