中国医学创新
中國醫學創新
중국의학창신
MEDICAL INNOVATION OF CHINA
2014年
15期
13-15,16
,共4页
实验性自身免疫性脑脊髓炎%多发性硬化%黏膜相关淋巴组织淋巴瘤转运蛋白1%白介素17
實驗性自身免疫性腦脊髓炎%多髮性硬化%黏膜相關淋巴組織淋巴瘤轉運蛋白1%白介素17
실험성자신면역성뇌척수염%다발성경화%점막상관림파조직림파류전운단백1%백개소17
Experimental allergic encephalomyelitis%Multiple Sclerosis%Mucosa associated lymphoid tissue lymphoma transport protein 1%Interleukin-17
目的：通过建立实验性自身免疫性脑脊髓炎（EAE）小鼠模型，观察小鼠脑组织中黏膜相关淋巴组织淋巴瘤转运蛋白1与血清白介素-17（Interleukin-17）的表达情况，并探讨两者在EAE发病机制中的作用。进一步探究MALTI是否可以作为多发性硬化（MS）的治疗靶点，以便寻找治疗MS的新手段。方法：将36只C57BL/6雌性小鼠按照随机数字表法分为EAE组和佐剂组各18只，EAE组采用MOG与完全弗氏佐剂的沫状混合物制备EAE模型，佐剂组仅给予弗氏佐剂。观察每只实验动物发病情况并每隔2 d进行一次神经功能评分。分别于免疫后14 d、24 d、40 d两组小鼠随机各取6只，心脏取血并4%多聚甲醛灌注取脑组织。取小鼠脑组织石蜡切片，免疫组化方法检测各组小鼠脑组织中MALT1并用ELISA法检测血清IL-17表达。结果：EAE组与佐剂组的小鼠神经功能评分、体重、血清中IL-17含量和脑组织中MALT1蛋白阳性细胞数比较差异均有统计学意义（P<0.05）。EAE组中24 d组小鼠的神经功能评分（2.50±0.55）分明显高于14 d组的（0.83±0.41）分和40 d组的（1.50±0.32）分，体重（17.04±0.41）g明显少于14 d组的（18.33±0.49）g和40 d组的（18.15±0.13）g，且血清中IL-17含量（288.00±26.45）pg/mL明显高于14 d组的（122.60±10.11）pg/mL和40 d组的（184.40±29.51）pg/mL，脑组织中MALT1蛋白阳性细胞数（183.80±9.25）个明显多于14 d组的（78.25±9.47）个和40 d组的（133.50±19.87）个，差异均有统计学意义（P<0.05）。结论：EAE小鼠神经功能评分越高，血清中IL-17含量越高，MALT1表达也越多，提示MALT1参与了EAE小鼠的发病过程，其机制可能与MALT1在EAE小鼠上调血清IL-17表达有关。
目的：通過建立實驗性自身免疫性腦脊髓炎（EAE）小鼠模型，觀察小鼠腦組織中黏膜相關淋巴組織淋巴瘤轉運蛋白1與血清白介素-17（Interleukin-17）的錶達情況，併探討兩者在EAE髮病機製中的作用。進一步探究MALTI是否可以作為多髮性硬化（MS）的治療靶點，以便尋找治療MS的新手段。方法：將36隻C57BL/6雌性小鼠按照隨機數字錶法分為EAE組和佐劑組各18隻，EAE組採用MOG與完全弗氏佐劑的沫狀混閤物製備EAE模型，佐劑組僅給予弗氏佐劑。觀察每隻實驗動物髮病情況併每隔2 d進行一次神經功能評分。分彆于免疫後14 d、24 d、40 d兩組小鼠隨機各取6隻，心髒取血併4%多聚甲醛灌註取腦組織。取小鼠腦組織石蠟切片，免疫組化方法檢測各組小鼠腦組織中MALT1併用ELISA法檢測血清IL-17錶達。結果：EAE組與佐劑組的小鼠神經功能評分、體重、血清中IL-17含量和腦組織中MALT1蛋白暘性細胞數比較差異均有統計學意義（P<0.05）。EAE組中24 d組小鼠的神經功能評分（2.50±0.55）分明顯高于14 d組的（0.83±0.41）分和40 d組的（1.50±0.32）分，體重（17.04±0.41）g明顯少于14 d組的（18.33±0.49）g和40 d組的（18.15±0.13）g，且血清中IL-17含量（288.00±26.45）pg/mL明顯高于14 d組的（122.60±10.11）pg/mL和40 d組的（184.40±29.51）pg/mL，腦組織中MALT1蛋白暘性細胞數（183.80±9.25）箇明顯多于14 d組的（78.25±9.47）箇和40 d組的（133.50±19.87）箇，差異均有統計學意義（P<0.05）。結論：EAE小鼠神經功能評分越高，血清中IL-17含量越高，MALT1錶達也越多，提示MALT1參與瞭EAE小鼠的髮病過程，其機製可能與MALT1在EAE小鼠上調血清IL-17錶達有關。
목적：통과건립실험성자신면역성뇌척수염（EAE）소서모형，관찰소서뇌조직중점막상관림파조직림파류전운단백1여혈청백개소-17（Interleukin-17）적표체정황，병탐토량자재EAE발병궤제중적작용。진일보탐구MALTI시부가이작위다발성경화（MS）적치료파점，이편심조치료MS적신수단。방법：장36지C57BL/6자성소서안조수궤수자표법분위EAE조화좌제조각18지，EAE조채용MOG여완전불씨좌제적말상혼합물제비EAE모형，좌제조부급여불씨좌제。관찰매지실험동물발병정황병매격2 d진행일차신경공능평분。분별우면역후14 d、24 d、40 d량조소서수궤각취6지，심장취혈병4%다취갑철관주취뇌조직。취소서뇌조직석사절편，면역조화방법검측각조소서뇌조직중MALT1병용ELISA법검측혈청IL-17표체。결과：EAE조여좌제조적소서신경공능평분、체중、혈청중IL-17함량화뇌조직중MALT1단백양성세포수비교차이균유통계학의의（P<0.05）。EAE조중24 d조소서적신경공능평분（2.50±0.55）분명현고우14 d조적（0.83±0.41）분화40 d조적（1.50±0.32）분，체중（17.04±0.41）g명현소우14 d조적（18.33±0.49）g화40 d조적（18.15±0.13）g，차혈청중IL-17함량（288.00±26.45）pg/mL명현고우14 d조적（122.60±10.11）pg/mL화40 d조적（184.40±29.51）pg/mL，뇌조직중MALT1단백양성세포수（183.80±9.25）개명현다우14 d조적（78.25±9.47）개화40 d조적（133.50±19.87）개，차이균유통계학의의（P<0.05）。결론：EAE소서신경공능평분월고，혈청중IL-17함량월고，MALT1표체야월다，제시MALT1삼여료EAE소서적발병과정，기궤제가능여MALT1재EAE소서상조혈청IL-17표체유관。
Objective:To observe the expression of Interleukin-17 in serum andMALT1 in brain tissue of mice through the establishment of experimental autoimmune encephalomyelitis(EAE)model in mice,andto explore their roles in the pathogenesis of EAE.To further explore whether MALTI can be usedin multiple sclerosis(MS)therapeutic target in order to findnew ways for the treatment of MS.Method:36 C57BL/6 female mice were randomly dividedinto the EAE model group andthe adjuvant group,18 rats in each group.The EAE model group was made the EAE model using MOG andfoam-like mixture of complete Freund’s adjuvant,the adjuvant group was only given Freund’s adjuvant. Each experimental animal infection situation was observed,andevery two days for a neurological function score.Six mice were selectedfrom two groups randomly after immunization 14 days,24 days,40 days,got heart blood,pouredinto 4%paraformaldehyde andremovedthe brain tissue.Took paraffin sections of brain tissue from mice,detectedMALT1 in the mouse brain tissue by immunohistochemical methodandIL-17 expression in serum by ELISA.Result:There were statistically significant differences in the nerve function score of mice,weight,the content of IL-17 in serum andMALT1 protein positive cells in the brain between the EAE model group andthe adjuvant group(P<0.05).The neurological function score of 24 dgroup in EAE model group was(2.50±0.55)score,it was significantly higher than the(0.83±0.41)score in 14 dgroup and(1.50±0.32)score in 40 dgroup,the weight of 24 dgroup in EAE model group was(17.04±0.41)g, it was significantly less than(18.33±0.49)g in 14 dgroup and(18.15±0.13)g in 40 dgroup,the content of IL-17 in serum of 24 dgroup in EAE model group was(288.00±26.45)pg/mL,it was significantly higher than(122.60±10.11)pg/mL in 14 dgroup and(184.40±29.51)pg/mL in 40 dgroup,andMALT1 protein positive cells in the brain of 24 dgroup in EAE model group was(183.80±9.25),it was significantly more than(78.25±9.47)in 14 dgroup and(133.50±19.87) in 40 dgroup,the differences were statistically significant(P<0.05).Conclusion:EAE mouse neural function score higher,the higher the content of serum IL-17,the higher the MALT1 expression.It shows that MALT1 is involvedin the S157.
