CAJ | 학술논문

　　目的：研究二烯丙基二硫（diallyl disulfide，DADS）通过Rac1-ADF/cofilin1通路对人结肠癌SW480细胞迁移与侵袭的作用。方法：划痕愈合和侵袭实验分别检测DADS对SW480细胞迁移与侵袭的影响；RT-PCR与Western blot检测DADS对SW480细胞Rac1-ADF/cofilin1信号分子表达的作用。结果：40与50 mg/L DADS处理SW480细胞24 h后，穿膜细胞分别减少57.12%与64.59%（P<0.05）。处理48 h细胞迁移率分别为23.23%与12.87%，较对照组75.86%明显降低（P<0.05）。RT-PCR显示，45 mg/L DADS处理SW480细胞24、48 h后，与对照组比较Rac1、Rock1、PAK1、LIMK1和destrin mRNA分别显著下调（P<0.01）；而cofilin1 mRNA无显著性差异（P>0.05）。Western blot显示，45 mg/L DADS处理SW480细胞6、12、24、48 h后，与对照组比较Rac1、Rock1、PAK1、LIMK1和destrin蛋白分别呈时间依赖性下调（P<0.05），但cofilin1蛋白无显著性差异（P>0.05），而p-LIMK1和p-cofilin1表达分别呈时间依赖性下调（P<0.05）。结论：DADS可通过Rac1-ADF/cofilin1通路下调Rac1、Rock1、PAK1、LIMK1、p-LIMK1、destrin与p-cofilin1抑制SW480细胞迁移与侵袭。
　　목적：연구이희병기이류（diallyl disulfide，DADS）통과Rac1-ADF/cofilin1통로대인결장암SW480세포천이여침습적작용。방법：화흔유합화침습실험분별검측DADS대SW480세포천이여침습적영향；RT-PCR여Western blot검측DADS대SW480세포Rac1-ADF/cofilin1신호분자표체적작용。결과：40여50 mg/L DADS처리SW480세포24 h후，천막세포분별감소57.12%여64.59%（P<0.05）。처리48 h세포천이솔분별위23.23%여12.87%，교대조조75.86%명현강저（P<0.05）。RT-PCR현시，45 mg/L DADS처리SW480세포24、48 h후，여대조조비교Rac1、Rock1、PAK1、LIMK1화destrin mRNA분별현저하조（P<0.01）；이cofilin1 mRNA무현저성차이（P>0.05）。Western blot현시，45 mg/L DADS처리SW480세포6、12、24、48 h후，여대조조비교Rac1、Rock1、PAK1、LIMK1화destrin단백분별정시간의뢰성하조（P<0.05），단cofilin1단백무현저성차이（P>0.05），이p-LIMK1화p-cofilin1표체분별정시간의뢰성하조（P<0.05）。결론：DADS가통과Rac1-ADF/cofilin1통로하조Rac1、Rock1、PAK1、LIMK1、p-LIMK1、destrin여p-cofilin1억제SW480세포천이여침습。
Objective:This work aims to investigate diallyl disulfide (DADS) inhibition of cell migration and invasion in human colon cancer SW480 cells through the Rac1-ADF/cofilin1 pathway. Methods:The potential of cell migration and invasion was examined by scratch healing assay and transwell membrane assay. The expression of Rac1-ADF/cofilin1 pathway was detected by RT-PCR and Western blot. Results:After the SW480 cells were treated with 40 and 50 mg·L-1 of DADS for 24 h, the number of transmembrane cells through the Matrigel obviously decreased by 57.12%and 64.59%, respectively (P<0.05). After cell treatment for 48 h, the cell migration rates were 23.23%and 12.87%, which were significantly lower compared with the control group (75.86%;P<0.05). After the cells were treated with 45 mg·L-1 of DADS for 24 and 48 h, the expression of Rac1, Rock1, PAK1, LIMK1, and destrin mRNA respectively decreased compared with the control group (P<0.05). However, no significant difference was observed in the expression of cofilin1 mRNA (P>0.05). After the treatment with 45 mg·L-1 of DADS for 6, 12, 24, and 48 h, the expression of Rac1, Rock1, PAK1, LIMK1, and Destrin proteins respectively decreased in a time-dependent manner compared with the control group (P<0.05). However, no significant differences were observed in the expression of the cofilin1 protein (P>0.05). Moreover, the expression of p-LIMK1 and p-cofilin1 notably decreased in a time-dependent manner (P<0.05). Conclusion:DADS inhibits cell migration and invasion, which is related to the down-regulation of Rac1, Rock1, PAK1, LIMK1, p-LIMK1, p-cofilin1, and destrin through the Rac1-ADF/cofilin1 pathway.