农业工程学报
農業工程學報
농업공정학보
2013年
15期
207-214
,共8页
龙焰%钟庄敏%尹华%林志勇%叶锦韶%何宝燕
龍燄%鐘莊敏%尹華%林誌勇%葉錦韶%何寶燕
룡염%종장민%윤화%림지용%협금소%하보연
甲烷%氧化%反硝化%填埋场覆土
甲烷%氧化%反硝化%填埋場覆土
갑완%양화%반초화%전매장복토
methane%oxidation%de-nitrification%landfill soil cover
垃圾填埋场的 CH4和渗滤液氮是两大污染因子。填埋覆土中因能进行 CH4氧化而具有削减填埋场 CH4排放的功能。同时，CH4可作为碳源促进反硝化。为此，该文研究了填埋场覆土中的 CH4好氧氧化-反硝化耦合（AME-D）特性，以期为填埋场同步强化控制CH4排放和氮污染提供依据。结果表明：CH4、O2和NO3--N均显著影响填埋覆土中的 CH4去除（p<0.05），三者影响的大小顺序为 CH4>O2>NO3--N，且 CH4和 O2具有交互作用（p<0.05）；CH4去除量随着初始CH4、O2体积分数的增大而增加，且与O2体积分数呈正相关关系（n=144，r=0.786， p<0.01）。CH4、O2和NO3--N明显影响CO2产生（p<0.01），且CH4和O2、O2和NO3--N均对CO2产生有交互作用（p<0.01）。CH4和O2对N2产生有明显影响（p<0.01），且两者有交互作用（p<0.01），NO3--N质量分数对N2产生影响不明显，但NO3--N和O2对N2产生有交互作用。低O2体积分数下（<5%），添加NO3--N能促进N2产生，高O2体积分数下（≥10%），NO3--N对N2产生影响不明显。C/O比对AME-D的影响与CH4和O2体积分数有关，比较合适的C/O比为0.5～1。该试验条件下，当CH4和、O2的体积分数分别为20%，NO3--N质量分数为100 mg/kg时，耦合效果最佳。该文可为垃圾填埋场CH4排放生物控制提供参考。
垃圾填埋場的 CH4和滲濾液氮是兩大汙染因子。填埋覆土中因能進行 CH4氧化而具有削減填埋場 CH4排放的功能。同時，CH4可作為碳源促進反硝化。為此，該文研究瞭填埋場覆土中的 CH4好氧氧化-反硝化耦閤（AME-D）特性，以期為填埋場同步彊化控製CH4排放和氮汙染提供依據。結果錶明：CH4、O2和NO3--N均顯著影響填埋覆土中的 CH4去除（p<0.05），三者影響的大小順序為 CH4>O2>NO3--N，且 CH4和 O2具有交互作用（p<0.05）；CH4去除量隨著初始CH4、O2體積分數的增大而增加，且與O2體積分數呈正相關關繫（n=144，r=0.786， p<0.01）。CH4、O2和NO3--N明顯影響CO2產生（p<0.01），且CH4和O2、O2和NO3--N均對CO2產生有交互作用（p<0.01）。CH4和O2對N2產生有明顯影響（p<0.01），且兩者有交互作用（p<0.01），NO3--N質量分數對N2產生影響不明顯，但NO3--N和O2對N2產生有交互作用。低O2體積分數下（<5%），添加NO3--N能促進N2產生，高O2體積分數下（≥10%），NO3--N對N2產生影響不明顯。C/O比對AME-D的影響與CH4和O2體積分數有關，比較閤適的C/O比為0.5～1。該試驗條件下，噹CH4和、O2的體積分數分彆為20%，NO3--N質量分數為100 mg/kg時，耦閤效果最佳。該文可為垃圾填埋場CH4排放生物控製提供參攷。
랄급전매장적 CH4화삼려액담시량대오염인자。전매복토중인능진행 CH4양화이구유삭감전매장 CH4배방적공능。동시，CH4가작위탄원촉진반초화。위차，해문연구료전매장복토중적 CH4호양양화-반초화우합（AME-D）특성，이기위전매장동보강화공제CH4배방화담오염제공의거。결과표명：CH4、O2화NO3--N균현저영향전매복토중적 CH4거제（p<0.05），삼자영향적대소순서위 CH4>O2>NO3--N，차 CH4화 O2구유교호작용（p<0.05）；CH4거제량수착초시CH4、O2체적분수적증대이증가，차여O2체적분수정정상관관계（n=144，r=0.786， p<0.01）。CH4、O2화NO3--N명현영향CO2산생（p<0.01），차CH4화O2、O2화NO3--N균대CO2산생유교호작용（p<0.01）。CH4화O2대N2산생유명현영향（p<0.01），차량자유교호작용（p<0.01），NO3--N질량분수대N2산생영향불명현，단NO3--N화O2대N2산생유교호작용。저O2체적분수하（<5%），첨가NO3--N능촉진N2산생，고O2체적분수하（≥10%），NO3--N대N2산생영향불명현。C/O비대AME-D적영향여CH4화O2체적분수유관，비교합괄적C/O비위0.5～1。해시험조건하，당CH4화、O2적체적분수분별위20%，NO3--N질량분수위100 mg/kg시，우합효과최가。해문가위랄급전매장CH4배방생물공제제공삼고。
Methane (CH4) is widely concerned because of its strong greenhouse effect. The CH4 generated from anaerobic biodegradation of waste in landfills is a significant source of atmospheric CH4. The CH4 generated transits the soil cover where it maybe be partly oxidized by CH4-oxidizing bacteria. Therefore, the landfill soil cover has a function on reducing CH4 emissions. On the other hand, N-pollution of leachate is the problem that must be controlled. According to documents, CH4 is a potentially inexpensive, widely available electron donor for biological denitrification of landfill leachate. Although no known methanotroph is able to denitrify, various consortia of microorganisms using methane as the sole carbon source which carry out denitrification both aerobically and anaerobically. Aerobic methane-oxidation coupled to denitrification (AME-D) is accomplished by aerobic methanotrophs oxidizing methane and releasing soluble organics that are used by coexisting denitrifiers as electron donors for denitrification. The work aimed to investigate the characteristics of AME-D in the cover soil of landfill. Thus, the batch assays were performed to investigate the effects of different factors such as CH4, O2 and NO3--N with different contents on AME-D. The concentration levels of CH4 were 0, 10, 20 and 30%, those of O2 were 0, 5,10 and 20%, and those of NO3--N were 0, 100, 200 and 300 mg/kg. In the assays, we first added 10 g of soil and NO3--N solution into 50 mL serum bottle with gas-tight rubber stoppers, then displaced the upper air in the bottle with argon gas and injected corresponding CH4 and O2 with syringe to make up the initial content, and finally incubated them at 30℃ for 7 d. The CH4, O2, CO2, N2 and N2O of gas samples in the third and seventh day were analyzed with a gas chromatograph (Fuli 9790, equipped with tandem connect of PorparkQ and 5? molecular sieve packed columns, and a thermal conductivity detector). Ar was used as carrier gas and its flow rate was 30 mL-1·min. Injector, oven, and detector temperatures were 50, 50, and 85℃, respectively. The results showed that the landfill cover soil was favorable for AME-D. The contents of CH4, O2 and NO3--N had significant influences on the CH4 oxidation (p<0.05, and followed the order of CH4>O2>NO3--N. Furthermore, the CH4 and O2 took significant interactions (p<0.05). The removal of CH4 increased as the increase of CH4 and O2, and was positively correlated with O2 concentration (n=144, r=0.786, p<0.01). The factor that had significant influences on the production of N2 was not NO3--N but CH4 and O2 (p<0.05). However, NO3--N and O2, CH4 and O2 took significant interactions (p<0.01) on the production of N2. The addition of NO3--N enhanced the product of N2 under low O2 content (<5%), while it took no effect under low O2 content (≥10%). The effects of C/O ratios on AME-D depended on the contents of O2 and CH4, the optimal range of C/O ratio was from 0.5 to 1.0. In the experiment, the AME-D coupled well as the contents of CH4, O2 and NO3--N were 20%, 20%and 100 mg/kg.
