CAJ | 학술논문

　　目的探讨2型糖尿病（T2DM ）遗传易感性与CAPN-10基因多态性的关系。方法采用限制性片段长度多态性聚合酶链反应（PCR-RFLP）技术对100例 T2DM 患者（病例组）和100例健康者（对照组）CAPN-10基因SNP19（rs3842570）、SNP43（rs3792267）和 SNP63（rs5030952）多态性位点进行基因分型。结果病例组CAPN-10基因43位点的GG基因型频率和G等位基因频率显著高于对照组，差异有统计学意义（P＜0．05）；19位点和63位点的基因型频率、等位基因频率在病例组与对照组分布差异均无统计学意义（P＞0．05）。结论CAPN-10基因SNP43（rs3842570）位点与T2DM的发生有相关性，而SNP19（rs3842570）和SNP63（rs5030952）位点则与T2DM 的发生无相关性。
　　목적탐토2형당뇨병（T2DM ）유전역감성여CAPN-10기인다태성적관계。방법채용한제성편단장도다태성취합매련반응（PCR-RFLP）기술대100례 T2DM 환자（병례조）화100례건강자（대조조）CAPN-10기인SNP19（rs3842570）、SNP43（rs3792267）화 SNP63（rs5030952）다태성위점진행기인분형。결과병례조CAPN-10기인43위점적GG기인형빈솔화G등위기인빈솔현저고우대조조，차이유통계학의의（P＜0．05）；19위점화63위점적기인형빈솔、등위기인빈솔재병례조여대조조분포차이균무통계학의의（P＞0．05）。결론CAPN-10기인SNP43（rs3842570）위점여T2DM적발생유상관성，이SNP19（rs3842570）화SNP63（rs5030952）위점칙여T2DM 적발생무상관성。
Objective To observe relationship between the genetic susceptibility and polymorphisms of CAPN-10 gene in type 2 diabetes mellitus (T2DM ) .Methods Restriction fragment length polymorphism polymerase chain reaction (PCR-RFLP) method was used to determine the distribution of allele and genotype frequencies of SNP 19 (rs3842570) ,SNP43 (rs3792267) and SNP63 (rs5030952) polymorphism in CAPN-10 gene in 100 T2DM patients (case group) and 100 normal control subjects (control group) .Results The frequencies of GG genotype and G allele of SNP43 in case group was significantly higher than that in control group (P< 0 .05) .There was no significant difference in genotypic frequencies and allele frequencies of SNP 19 and SNP63 between case group and control group (P>0 .05) .Conclusion The polymorphism of SNP43 in CAPN-10 gene may contribute to the genetic susceptibility to T2DM ,but SNP19 and SNP63 in CAPN-10 gene may be not related to T 2DM susceptibility possibly .