神经损伤与功能重建
神經損傷與功能重建
신경손상여공능중건
NEURAL INJURY AND FUNCTIONAL RECONSTRUCTION
2014年
3期
191-194
,共4页
闵喆%李昌盛%方瑜%邱占东%张苏明
閔喆%李昌盛%方瑜%邱佔東%張囌明
민철%리창성%방유%구점동%장소명
大鼠%大脑中动脉栓塞%激光散斑成像%侧枝循环
大鼠%大腦中動脈栓塞%激光散斑成像%側枝循環
대서%대뇌중동맥전새%격광산반성상%측지순배
rats%middle cerebral artery occlusion%laser speckle imaging%collateral blood flow
目的：利用激光散斑成像（LSI）技术观察大鼠大脑中动脉栓塞（MCAO）模型侧枝循环开放情况及局部脑血流的变化。方法：成年雄性 SD 大鼠9只，使用改良线栓法制备永久性大脑中动脉栓塞（pMCAO）模型。利用 LSI 技术观测术后90 min 内缺血半球皮质血流变化及侧枝循环开放情况，术后24 h TTC 染色法观察脑梗死体积。结果：造模成功后大鼠右侧大脑半球皮质大脑中动脉供血区血流明显减少，部分大鼠随着侧枝循环逐渐开放局部血流不同程度恢复，另一部分大鼠血流恢复缓慢。结论：LSI 技术时间空间分辨率高，成像结果直观。
目的：利用激光散斑成像（LSI）技術觀察大鼠大腦中動脈栓塞（MCAO）模型側枝循環開放情況及跼部腦血流的變化。方法：成年雄性 SD 大鼠9隻，使用改良線栓法製備永久性大腦中動脈栓塞（pMCAO）模型。利用 LSI 技術觀測術後90 min 內缺血半毬皮質血流變化及側枝循環開放情況，術後24 h TTC 染色法觀察腦梗死體積。結果：造模成功後大鼠右側大腦半毬皮質大腦中動脈供血區血流明顯減少，部分大鼠隨著側枝循環逐漸開放跼部血流不同程度恢複，另一部分大鼠血流恢複緩慢。結論：LSI 技術時間空間分辨率高，成像結果直觀。
목적：이용격광산반성상（LSI）기술관찰대서대뇌중동맥전새（MCAO）모형측지순배개방정황급국부뇌혈류적변화。방법：성년웅성 SD 대서9지，사용개량선전법제비영구성대뇌중동맥전새（pMCAO）모형。이용 LSI 기술관측술후90 min 내결혈반구피질혈류변화급측지순배개방정황，술후24 h TTC 염색법관찰뇌경사체적。결과：조모성공후대서우측대뇌반구피질대뇌중동맥공혈구혈류명현감소，부분대서수착측지순배축점개방국부혈류불동정도회복，령일부분대서혈류회복완만。결론：LSI 기술시간공간분변솔고，성상결과직관。
ObjectiveTo study the collateral blood flow and focal cerebral blood flow (CBF) in middle cerebral artery occlusion (MCAO) rats by laser speckle imaging (LSI). Methods: A permanent MCAO (pMCAO) model was generated in male SD rats (n=9) by the intraluminal filament technique. LSI was used to measure CBF and collateral blood flow in the ischemic cortical area and MCA territory after 90 min. The brain was stained with TTC to determine the infarct size 24 h later. Results: CBF was decreased in both the hemispheric cortical area and MCA territory in the first 15 min and increased blood flow velocity was observed in some animals later. Conclusion: LSI has high spatial and temporal resolution, and offers visual imaging.
