中国农学通报
中國農學通報
중국농학통보
CHINESE AGRICULTURAL SCIENCE BULLETIN
2013年
21期
126-130
,共5页
叶世超%薛婷%王晓斐%何文锦%叶冰莹%陈由强
葉世超%薛婷%王曉斐%何文錦%葉冰瑩%陳由彊
협세초%설정%왕효비%하문금%협빙형%진유강
耐高温酵母%耐高温机制%基因组改组技术
耐高溫酵母%耐高溫機製%基因組改組技術
내고온효모%내고온궤제%기인조개조기술
thermo-resistant yeast%thermo-resistant mechanism%genome shuffling
归纳了热激蛋白、海藻糖、应激糖蛋白及相关基因与酵母耐热性机制的关系,并对提高酿酒酵母耐热性的技术进行了综述,主要包括高温驯化、自然选育、诱变育种、原生质体融合育种及Genome shuffling技术,重点介绍了Genome shuffling技术的原理及其在选育耐高温酵母应用中的优势,该技术与传统微生物育种技术、现代生物技术相结合,将大大提高耐高温乙醇酵母菌种的选育效率。提出了提高酵母耐热性存在的问题,例如耐高温机制不明确和相关基因是如何产生和影响细胞温度耐受性的认识还很有限等。同时对研究前景进行了展望。
歸納瞭熱激蛋白、海藻糖、應激糖蛋白及相關基因與酵母耐熱性機製的關繫,併對提高釀酒酵母耐熱性的技術進行瞭綜述,主要包括高溫馴化、自然選育、誘變育種、原生質體融閤育種及Genome shuffling技術,重點介紹瞭Genome shuffling技術的原理及其在選育耐高溫酵母應用中的優勢,該技術與傳統微生物育種技術、現代生物技術相結閤,將大大提高耐高溫乙醇酵母菌種的選育效率。提齣瞭提高酵母耐熱性存在的問題,例如耐高溫機製不明確和相關基因是如何產生和影響細胞溫度耐受性的認識還很有限等。同時對研究前景進行瞭展望。
귀납료열격단백、해조당、응격당단백급상관기인여효모내열성궤제적관계,병대제고양주효모내열성적기술진행료종술,주요포괄고온순화、자연선육、유변육충、원생질체융합육충급Genome shuffling기술,중점개소료Genome shuffling기술적원리급기재선육내고온효모응용중적우세,해기술여전통미생물육충기술、현대생물기술상결합,장대대제고내고온을순효모균충적선육효솔。제출료제고효모내열성존재적문제,례여내고온궤제불명학화상관기인시여하산생화영향세포온도내수성적인식환흔유한등。동시대연구전경진행료전망。
The relationship between heat shock proteins, trehalose, stress glycoprotein, related gene and yeast heat resistance mechanism were summarized in this paper. Beside, the research progress in the breeding of heat-resistance yeast by high temperature domestication, natural breeding, mutation breeding, protoplast infusion and genome shuffling was reviewed, especially the theory of genome shuffling techniques and its advantages in the breeding of heat-resistance yeast were induced. The problems were put forward in research of the breeding of heat-resistance yeast. For example, high temperature resistant mechanism was not explicit and the cognize of related gene how to generate and impact cell temperature tolerance are limited. Meanwhile, the research prospect was prospected.