CAJ | 학술논문

　　为了从有机磷农药严重污染的土壤中分离筛选出一株有机磷农药高效降解菌，并对其进行鉴定及其酶学性质研究。通过有机磷农药驯化培养、形态学和生理生化特性及其16S rDNA序列分析等试验对其进行了初步鉴定，并初步研究了pH值、温度、底物及金属离子等外界因素对其粗酶液酶学性质的影响。分离筛选出一株有机磷农药高效降解菌J7-4，经形态学和生理生化特性及其16S rDNA序列分析等试验，初步将其鉴定为施氏假单胞菌(Pseudomonas stutzeri)，其降解酶为胞内酶，该酶最适pH为9.0，最适反应温度为45℃；最适底物为甲基对硫磷，并且该酶具有广谱降解特性；Mg2+、Ca2+等金属离子对其有不同程度的激活作用，Co2+、Cr3+对该酶有轻微的抑制作用，而Mn2+、Fe3+、Zn2+、Al3+、Hg+、Cd2+、Ba2+、Ag+对该酶有强烈的抑制作用。成功地从有机磷农药严重污染的土壤中分离筛选出了一株有机磷农药高效降解菌J7-4，并对其降解酶的性质进行了初步研究。
　　위료종유궤린농약엄중오염적토양중분리사선출일주유궤린농약고효강해균，병대기진행감정급기매학성질연구。통과유궤린농약순화배양、형태학화생리생화특성급기16S rDNA서렬분석등시험대기진행료초보감정，병초보연구료pH치、온도、저물급금속리자등외계인소대기조매액매학성질적영향。분리사선출일주유궤린농약고효강해균J7-4，경형태학화생리생화특성급기16S rDNA서렬분석등시험，초보장기감정위시씨가단포균(Pseudomonas stutzeri)，기강해매위포내매，해매최괄pH위9.0，최괄반응온도위45℃；최괄저물위갑기대류린，병차해매구유엄보강해특성；Mg2+、Ca2+등금속리자대기유불동정도적격활작용，Co2+、Cr3+대해매유경미적억제작용，이Mn2+、Fe3+、Zn2+、Al3+、Hg+、Cd2+、Ba2+、Ag+대해매유강렬적억제작용。성공지종유궤린농약엄중오염적토양중분리사선출료일주유궤린농약고효강해균J7-4，병대기강해매적성질진행료초보연구。
The aims were to screen a high-efficiency biodegradating strains from the contaminated soil and identify its enzymology properties. In this study, A high-efficiency organophosphorus-degrading strain was selected through domestication, separation, screening process and identified by a series of colony morphological, physiological characteristics and 16s rDNA sequences and other tests. And the crude enzyme liquid enzymatic properties were studied by the step of pH, temperature of the enzyme, metal ions and etc. A strain J7-4 was screened and identified as Pseudomonas stutzeri. The results showed that the degradation enzyme was located in the cell. The optional reactive pH and temperature of the enzyme was 45℃and pH 9.0. The most suitable substrate of the enzyme was methyl parathion, and it had a broad-spectrum degradation characteristics. Some metal ions such as Mg2+, Ca2+had activation on the enzyme at different degree, while Co2+, Cr3+had slight inhibition on the enzyme, and Mn2+, Fe3+, Zn2+, Al3+, Hg+, Cd2+, Ba2+, Ag+had a strong inhibition on the enzyme. A strain Pseudomonas stutzeri J7-4 was screened successfully with high efficient degradating organophosphate compounds and its enzymology properties were studied.