CAJ | 학술논문

　　构建苦荞遗传连锁图谱，为今后有关苦荞基因组结构、重要农艺性状QTL定位、分子标记辅助育种和基因克隆等研究工作奠定基础。以栽培苦荞‘滇宁一号’和苦荞野生近缘种杂交产生的119份F4代分离材料为作图群体，利用SSR分子标记来构建苦荞的分子遗传连锁图谱。本研究构建的连锁图谱包含15个连锁群，由89个标记组成，其中偏分离的标记有22个，占24.7%，每条连锁群上的标记在2~16之间。连锁群长度在6.9~165.8 cM的范围，覆盖基因组860.2 cM，总平均长度9.7 cM。本研究构建了首张苦荞SSR遗传连锁图谱，为苦荞QTL定位、基因克隆、遗传选育等研究奠定了基础。
　　구건고교유전련쇄도보，위금후유관고교기인조결구、중요농예성상QTL정위、분자표기보조육충화기인극륭등연구공작전정기출。이재배고교‘전저일호’화고교야생근연충잡교산생적119빈F4대분리재료위작도군체，이용SSR분자표기래구건고교적분자유전련쇄도보。본연구구건적련쇄도보포함15개련쇄군，유89개표기조성，기중편분리적표기유22개，점24.7%，매조련쇄군상적표기재2~16지간。련쇄군장도재6.9~165.8 cM적범위，복개기인조860.2 cM，총평균장도9.7 cM。본연구구건료수장고교SSR유전련쇄도보，위고교QTL정위、기인극륭、유전선육등연구전정료기출。
The present study was aimed to construct a molecular linkage map of tartary buckwheat, which would provide essential tools for investigating genomic structure and evolution, and facilitate QTL mapping, marker-assisted selection and map-based cloning of important gene. In this study, a population of F4 generation including 119 individual plants was derived from the cross between‘Dianning 1’and a tartary buckwheat wild species. SSR markers were used to construct the molecular genetic linkage map. The genetic linkage map consisted 15 linkage groups with 89 markers including 22 (24.7%) distorted markers in the whole map and 2-16 markers in each linkage group. The total length of the map was 860.2 cM. The length of linkage groups varied from 6.9 to 165.8 cM, with a mean marker interval distance of 9.7 cM between markers. The current study constructed a first tartary buckwheat genetic linkage map, which provided the basis for the research on gene location and molecular breeding of tartary buckwheat.