CAJ | 학술논문

　　SSR标记具有快速、稳定、准确的技术优势，已经在品种鉴定等方面得到广泛的应用。为了鉴定生产用“两杂”种子样品的遗传多样性及纯度，利用36对SSR引物对39份杂交水稻样品进行了遗传多样性鉴定，其中2个样品为“同名异种”（遗传距离为0.11），有2个样品疑似“异名同种”；利用33对SSR引物对36份杂交玉米样品进行了遗传多样性鉴定，同样存在“同名异种”和“异名同种”现象。在人为混杂的杂交水稻样本中，SSR标记检测值与实际混杂率u测验没有显著性差异；同时利用SSR标记和田间鉴定方法比较种子公司提供的10个杂交水稻样品纯度，尽管有3个样品SSR标记检测结果低于田间鉴定，但由于田间纯度鉴定以农艺性状为标准，遗传背景相似的单株难以区分，因此从加强种子质量监管角度考虑，SSR分子标记鉴定技术更为有利。
　　SSR표기구유쾌속、은정、준학적기술우세，이경재품충감정등방면득도엄범적응용。위료감정생산용“량잡”충자양품적유전다양성급순도，이용36대SSR인물대39빈잡교수도양품진행료유전다양성감정，기중2개양품위“동명이충”（유전거리위0.11），유2개양품의사“이명동충”；이용33대SSR인물대36빈잡교옥미양품진행료유전다양성감정，동양존재“동명이충”화“이명동충”현상。재인위혼잡적잡교수도양본중，SSR표기검측치여실제혼잡솔u측험몰유현저성차이；동시이용SSR표기화전간감정방법비교충자공사제공적10개잡교수도양품순도，진관유3개양품SSR표기검측결과저우전간감정，단유우전간순도감정이농예성상위표준，유전배경상사적단주난이구분，인차종가강충자질량감관각도고필，SSR분자표기감정기술경위유리。
SSR molecular markers which had the technical advantages of fast, stable and accurate were used to investigate the genetic diversity and purity test of two hybrid seed commercially available. 36 rice SSR primer pairs were used to assess the genetic diversity of 38 hybrid rice samples. The result showed two of the sample were heterogenous (genetic distance 0.11), whereas two of the others seemed to be synonym (genetic distance 0.03). 33 maize SSR primer pairs were used to determine the genetic diversity of 36 hybrid maize sample, the result showed two of the sample were heterogenous (genetic distance 0.12). In the artificial mixed sample test, u test revealed there was no significant difference between all sample test value and actual mixed ratio. Meanwhile, 10 hybrid rice samples provided by seed company were compared with SSR molecular marker and field identification methods, though SSR marker test results were below the field identification in three samples, the field identifying were on the basis of agronomic traits, it was difficult to distinguish the similar genetic background strains, it indicated that SSR molecular marker was more favorable than the other detection methods.