CAJ | 학술논문

目的：探索组织工程骨膜体内成骨修复兔大段骨缺损的可行性。方法培养新西兰大白兔骨髓间充质干细胞（BMSCs），以成骨诱导剂诱导成骨分化后，与猪小肠粘膜下层（SIS）复合构建组织工程骨膜。扫描电镜（SEM）观察细胞与材料复合情况。选4月龄新西兰大白兔24只，制备单侧桡骨干4 cm缺损模型。随机选12只植入组织工程骨膜，作为实验组；另12只骨缺损旷置，作为对照组。术后6周后摄X线片观察，切取整段桡骨作为标本行HE及Masson染色观察。结果 BMSCs诱导14 d后可成骨分化。SEM显示构建的组织工程骨膜上黏附大量种子细胞。X线片观察：实验组骨缺损处有长柱状新生骨形成，并与截骨端骨性融合，密度与正常骨相近；对照组骨缺损处无成骨征象，密度同周围软组织影。组织学观察：实验组骨缺损处有新骨形成，新生骨组织中可见丰富的血管腔及不规则髓腔样结构；对照组骨缺损处仅为纤维结缔组织，无骨组织形成。结论以SIS和BMSCs构建的组织工程骨膜有修复兔大段骨缺损的可行性。组织工程骨膜有进一步深入研究、开发的价值和前景。
목적：탐색조직공정골막체내성골수복토대단골결손적가행성。방법배양신서란대백토골수간충질간세포（BMSCs），이성골유도제유도성골분화후，여저소장점막하층（SIS）복합구건조직공정골막。소묘전경（SEM）관찰세포여재료복합정황。선4월령신서란대백토24지，제비단측뇨골간4 cm결손모형。수궤선12지식입조직공정골막，작위실험조；령12지골결손광치，작위대조조。술후6주후섭X선편관찰，절취정단뇨골작위표본행HE급Masson염색관찰。결과 BMSCs유도14 d후가성골분화。SEM현시구건적조직공정골막상점부대량충자세포。X선편관찰：실험조골결손처유장주상신생골형성，병여절골단골성융합，밀도여정상골상근；대조조골결손처무성골정상，밀도동주위연조직영。조직학관찰：실험조골결손처유신골형성，신생골조직중가견봉부적혈관강급불규칙수강양결구；대조조골결손처부위섬유결체조직，무골조직형성。결론이SIS화BMSCs구건적조직공정골막유수복토대단골결손적가행성。조직공정골막유진일보심입연구、개발적개치화전경。
Objective To explore the feasibility of rabbit large segmental bone defects repaired by using tissue engineered periosteum.Methods Rabbit bone marrow mesenchymal stem cells (BMSCs)were cultured and osteogenetically induced in-to osteoblasts.Porcine small intestine submucosa (SIS)scaffold was produced by decellular and a series mechanical and physiochemical procedures.Then tissue engineered periosteum was constructed by combining osteogenic BMSCs and SIS.A 4cm length of radius with natural periosteum around was osteotomied to create large segmental bone defect model in 24 4-month-old New Zealand white rabbits.Then the animals were randomly divided into 2 equal groups.In experimental group, defect area was occupied by tissue engineered periosteum with a shape of capsular tube which was created by suturing the film circling around the both bone ends,while in control group,the defect was untreated.After Six weeks,the large segmen-tal bone defects were checked by X-ray and histological evaluation.Results BMSCs were differentiated into osteoblasts in 14 days.SEM showed abundant seeding cells adhered on tissue engineered periosteum .In experimental group,new bone tissue was formed and bridged the defect gap,of which could be confirmed by x-ray film and histological staining.There exists a lot of newly formed vessels and medullary cavity among of the new bone tissue,but without lymphocytes infiltration.While in the control group,no any osteogenesis either in X ray,or in histological staining inspecting,and the defect space only re-placed by some connective tissue.Conclusion Tissue engineered periosteum has the feasibility to repair the large diaphysis defect.Tissue engineered periosteum is worthy of the further investigation and exploitation for clinic.