石河子大学学报（自然科学版）
石河子大學學報（自然科學版）
석하자대학학보（자연과학판）
JOURNAL OF SHIHEZI UNIVERSITY (NATURAL SCIENCE)
2014年
2期
169-174
,共6页
庄睿%姜晓霞%张峰%梁晨%樊超%李文娟%吴芳%章乐%吴江东%张春军%张辉%张万江
莊睿%薑曉霞%張峰%樑晨%樊超%李文娟%吳芳%章樂%吳江東%張春軍%張輝%張萬江
장예%강효하%장봉%량신%번초%리문연%오방%장악%오강동%장춘군%장휘%장만강
结核分枝杆菌%小鼠肺泡巨噬细胞%转铁蛋白受体%铁蛋白q
結覈分枝桿菌%小鼠肺泡巨噬細胞%轉鐵蛋白受體%鐵蛋白q
결핵분지간균%소서폐포거서세포%전철단백수체%철단백q
Mycobacterium tuberculosis%Mouse alveolar macrophages%Transferrin receptor%Ferritin
探讨不同毒力结核分枝杆菌感染对小鼠肺泡巨噬细胞转铁蛋白受体（TfR）和铁蛋白（Fn）表达的影响及其时相性变化。利用制备的卡介苗(以下简称BCG)和结核分枝杆菌国际标准强毒株H37Rv株（以下简称H37Rv株）悬液，分别经小鼠尾静脉注射，建立各组小鼠感染模型，各组小鼠感染模型建立成功后，分别于第1、3、5、7、9、11、13、15天，进行各组小鼠肺泡灌洗，收集小鼠肺泡灌洗液，获取各组小鼠肺泡巨噬细胞。应用ELISA方法和Western blot技术检测各组小鼠肺泡巨噬细胞TfR和Fn的表达。利用ELISA方法检测各组小鼠肺泡巨噬细胞TfR的表达结果显示：H37RV组与BCG组小鼠肺泡巨噬细胞TfR表达均高于空白对照组，在感染第7、9、11天差异最明显，具有统计学意义(P<0.05)。利用Western blot技术检测各组小鼠肺泡巨噬细胞TfR表达结果显示：于模型建成后第7、9、11天，H37RN组、BCG组、空白对照组三组之间差异有统计学意义(P<0.05)。用ELISA方法和Western blot技术检测各组小鼠肺泡巨噬细胞内Fn表达结果显示：于模型建成后第7、9、11天，H37RV组与BCG组的小鼠肺泡巨噬细胞内Fn表达量明显减低，并且于第7天时表达量最第，异有统计学意义(P<0.05)。结核分枝杆菌感染小鼠，导致小鼠肺泡巨噬细胞TfR的表达增高，而Fn的表达降低。不同毒力的结核杆菌感染后Fn蛋白表达差异并不明显,而不同毒力的结核杆菌感染后TfR蛋白的表达有差异性。
探討不同毒力結覈分枝桿菌感染對小鼠肺泡巨噬細胞轉鐵蛋白受體（TfR）和鐵蛋白（Fn）錶達的影響及其時相性變化。利用製備的卡介苗(以下簡稱BCG)和結覈分枝桿菌國際標準彊毒株H37Rv株（以下簡稱H37Rv株）懸液，分彆經小鼠尾靜脈註射，建立各組小鼠感染模型，各組小鼠感染模型建立成功後，分彆于第1、3、5、7、9、11、13、15天，進行各組小鼠肺泡灌洗，收集小鼠肺泡灌洗液，穫取各組小鼠肺泡巨噬細胞。應用ELISA方法和Western blot技術檢測各組小鼠肺泡巨噬細胞TfR和Fn的錶達。利用ELISA方法檢測各組小鼠肺泡巨噬細胞TfR的錶達結果顯示：H37RV組與BCG組小鼠肺泡巨噬細胞TfR錶達均高于空白對照組，在感染第7、9、11天差異最明顯，具有統計學意義(P<0.05)。利用Western blot技術檢測各組小鼠肺泡巨噬細胞TfR錶達結果顯示：于模型建成後第7、9、11天，H37RN組、BCG組、空白對照組三組之間差異有統計學意義(P<0.05)。用ELISA方法和Western blot技術檢測各組小鼠肺泡巨噬細胞內Fn錶達結果顯示：于模型建成後第7、9、11天，H37RV組與BCG組的小鼠肺泡巨噬細胞內Fn錶達量明顯減低，併且于第7天時錶達量最第，異有統計學意義(P<0.05)。結覈分枝桿菌感染小鼠，導緻小鼠肺泡巨噬細胞TfR的錶達增高，而Fn的錶達降低。不同毒力的結覈桿菌感染後Fn蛋白錶達差異併不明顯,而不同毒力的結覈桿菌感染後TfR蛋白的錶達有差異性。
탐토불동독력결핵분지간균감염대소서폐포거서세포전철단백수체（TfR）화철단백（Fn）표체적영향급기시상성변화。이용제비적잡개묘(이하간칭BCG)화결핵분지간균국제표준강독주H37Rv주（이하간칭H37Rv주）현액，분별경소서미정맥주사，건립각조소서감염모형，각조소서감염모형건립성공후，분별우제1、3、5、7、9、11、13、15천，진행각조소서폐포관세，수집소서폐포관세액，획취각조소서폐포거서세포。응용ELISA방법화Western blot기술검측각조소서폐포거서세포TfR화Fn적표체。이용ELISA방법검측각조소서폐포거서세포TfR적표체결과현시：H37RV조여BCG조소서폐포거서세포TfR표체균고우공백대조조，재감염제7、9、11천차이최명현，구유통계학의의(P<0.05)。이용Western blot기술검측각조소서폐포거서세포TfR표체결과현시：우모형건성후제7、9、11천，H37RN조、BCG조、공백대조조삼조지간차이유통계학의의(P<0.05)。용ELISA방법화Western blot기술검측각조소서폐포거서세포내Fn표체결과현시：우모형건성후제7、9、11천，H37RV조여BCG조적소서폐포거서세포내Fn표체량명현감저，병차우제7천시표체량최제，이유통계학의의(P<0.05)。결핵분지간균감염소서，도치소서폐포거서세포TfR적표체증고，이Fn적표체강저。불동독력적결핵간균감염후Fn단백표체차이병불명현,이불동독력적결핵간균감염후TfR단백적표체유차이성。
Objective to discuss the phase transformation and it's significance of the transferrin receptor (TfR) in macrophages and the expression quantity of ferritin (Fn) when the mice infected by different virulence of mycobacterium tuberculosis. Methods:Through the tail intravenous injection the bacille calmette guerin (BCG) and Mycobacterium tuberculosis strains international standard virulent strain (H37Rv strain) would built various group infection model in mice,after the success of these kinds of injection model,the alveolar wash will be done at the first day,the third day,the fifth day,the ninth day,the thirteenth day and the fifteenth day,and later obtaining the alveolar macrophage after collected the alveolar lavage fluid of mice,then detect the expression quantity of TfR and Fn by means of the applying of ELISA and Western-blot.The result of detection showing that the expression quantity of TfR contained in the alveolar macrophage of mice which from the various groups is higher than other matched group,it's most obvious at the day of 7th,9th,11th,which possess the significance of Statistical (P<0.05).The detection of Western-blot showing that after the build of model at the day of 7th,9th,11th,the difference of the various infectious group possess the significance of Statistical (P<0.05),and the H37RV group is much higher the expression quantity of BCG proteome at the day of 7th, 9th,11th.Through the detection by ELISA and Western blot showing the expression quantity of Fn in alveolar macrophage from various groups, the expression quantity is the lest at the seventh day.the groups of H37RV and BCG had been reduced at the day of 7th,9th,11th which possess the significance of Statistical.Mycobacterium tuberculosis infection had brought about the expression quantity of TfR in Macrophages much higher.While the expression quantity of Fn had been reduced.It's not abvious between the difference of protein which infected by different virulence of mycobacterium tuberculosis.
