石河子大学学报（自然科学版）
石河子大學學報（自然科學版）
석하자대학학보（자연과학판）
JOURNAL OF SHIHEZI UNIVERSITY (NATURAL SCIENCE)
2014年
2期
133-137
,共5页
矮小突变体%转录组%大豆%GASA保守域
矮小突變體%轉錄組%大豆%GASA保守域
왜소돌변체%전록조%대두%GASA보수역
dwarf mutant%transcriptome%soybean%GASA domain
植株高度是影响农作物产量的重要性状，本实验以大豆矮小突变体为研究材料，发现GA3处理可以部分恢复其矮化表型。为了探究其至矮的分子机制，运用RNA-Seq技术测序分析了野生型和突变体的转录组差异，结果表明有多个与植物激素相关的基因差异表达，其中Glyma19g01590与植物激素GA调节有关，生物信息学分析表明差异表达基因Glyma19g01590的开放阅读框有594 bp，推测编码198个氨基酸，该蛋白具有GASA （Gibberellic Acid-Stimulated in Arabidopsis）保守区域，GASA保守区域与GA调节有关。半定量RT-PCR实验表明Glyma19g01590在突变体中的表达水平明显高于野生型，qRT-PCR实验表明Glyma19g01590在突变体的表达量大约是是野生型的24倍。根据以上结果推测Glyma19g01590基因是导致大豆矮小表型的因素之一。
植株高度是影響農作物產量的重要性狀，本實驗以大豆矮小突變體為研究材料，髮現GA3處理可以部分恢複其矮化錶型。為瞭探究其至矮的分子機製，運用RNA-Seq技術測序分析瞭野生型和突變體的轉錄組差異，結果錶明有多箇與植物激素相關的基因差異錶達，其中Glyma19g01590與植物激素GA調節有關，生物信息學分析錶明差異錶達基因Glyma19g01590的開放閱讀框有594 bp，推測編碼198箇氨基痠，該蛋白具有GASA （Gibberellic Acid-Stimulated in Arabidopsis）保守區域，GASA保守區域與GA調節有關。半定量RT-PCR實驗錶明Glyma19g01590在突變體中的錶達水平明顯高于野生型，qRT-PCR實驗錶明Glyma19g01590在突變體的錶達量大約是是野生型的24倍。根據以上結果推測Glyma19g01590基因是導緻大豆矮小錶型的因素之一。
식주고도시영향농작물산량적중요성상，본실험이대두왜소돌변체위연구재료，발현GA3처리가이부분회복기왜화표형。위료탐구기지왜적분자궤제，운용RNA-Seq기술측서분석료야생형화돌변체적전록조차이，결과표명유다개여식물격소상관적기인차이표체，기중Glyma19g01590여식물격소GA조절유관，생물신식학분석표명차이표체기인Glyma19g01590적개방열독광유594 bp，추측편마198개안기산，해단백구유GASA （Gibberellic Acid-Stimulated in Arabidopsis）보수구역，GASA보수구역여GA조절유관。반정량RT-PCR실험표명Glyma19g01590재돌변체중적표체수평명현고우야생형，qRT-PCR실험표명Glyma19g01590재돌변체적표체량대약시시야생형적24배。근거이상결과추측Glyma19g01590기인시도치대두왜소표형적인소지일。
Plant height is an important agronomic character for crop yield improvement.In this study a dwarf mutant could be partially rescued treated by GA3.To explore the molecular mechanism of leading to dwarf phenotype,RNA-seq was used to identify different genes between mutant and wild type,and many genes related to phytohormone were detected to be differential expressed, in which Glyma19g01590 gene expression relates to GA.Bioinformatics analysis indicated that Glyma19g01590 gene contained 594 bp in open reading frame (ORF) and presumably encoded 198 amino acids.Glyma19g01590 protein contains a GASA (Gibberellic Acid-Stimulated in Arabidopsis) domain which could be regulated by GA3.In addition,semi-quantitative RT-PCR experiment showed that the expression level of Glyma19g01590 gene in mutant was higher than that in wild type. Quantitative Real-time RT-PCR (qRT-PCR) result showed the expression quantity of Glyma19g01590 gene in Gmdwarf1 was 24 times compared with that in wild type.The results indicated that Glyma19g01590 gene was perhaps one of the genes that are responsible for dwarf phenotype.
