CAJ | 학술논문

　　旨在筛选出分离纯化虎杖中白藜芦醇的最佳大孔吸附树脂以及工艺条件。静态吸附与解吸、动态吸附与解吸相结合的方法，以树脂的最大吸附量、解吸率为考察指标，确定最佳的纯化工艺。H103树脂对白藜芦醇有较好的吸附与解吸效果，其最佳的工艺条件为粗提液中白藜芦醇的质量浓度为0.72 mg/mL、上样流速2 BV/h，吸附饱和后先用4 BV的蒸馏水进行洗涤，然后用8 BV、75%的乙醇溶液以1.5 BV/h的流速进行洗脱，白藜芦醇的含量可由纯化前的12.8%提升至53.5%。应用H103树脂对虎杖中的白藜芦醇进行纯化，其工艺稳定可行，具有吸附快、解吸容易、解吸液安全低毒且回收容易的特点，具有较高的应用价值。
　　지재사선출분리순화호장중백려호순적최가대공흡부수지이급공예조건。정태흡부여해흡、동태흡부여해흡상결합적방법，이수지적최대흡부량、해흡솔위고찰지표，학정최가적순화공예。H103수지대백려호순유교호적흡부여해흡효과，기최가적공예조건위조제액중백려호순적질량농도위0.72 mg/mL、상양류속2 BV/h，흡부포화후선용4 BV적증류수진행세조，연후용8 BV、75%적을순용액이1.5 BV/h적류속진행세탈，백려호순적함량가유순화전적12.8%제승지53.5%。응용H103수지대호장중적백려호순진행순화，기공예은정가행，구유흡부쾌、해흡용역、해흡액안전저독차회수용역적특점，구유교고적응용개치。
Various macroporous resins with different properties were employed to purify the resveratrol from the roots of Polygonum cuspidatum. Static and dynamic adsorption and desorption experiments were conducted, meanwhile, the adsorption and desorption rates of resveratrol were concerned to test out the prominent macroporous resin and optimal processing parameters. H103 was subsequently selected as the best macroporous resin. The optimal processing parameters were obtained as follows: the crude extract containing resveratrol of 0.72 mg/mL was loaded at a flow rate of 2 BV/h, the H103 column was washed with 4 BV of distilled water for removing the impurities, and elution with 8 BV of 75% ethanol was at a flow rate of 1.5 BV/h. As a result, the content of purified resveratrol was increased from 12.8%to 53.5%. The method with H103 as the optimal macroporous resin undergoing with the modified purification process is a simple, safe and inexpensive technology, and has a promising potential of purifying resveratrol from the roots of Polygonum cuspidatum.