CAJ | 학술논문

　　为了研究木质素代谢途径中相关酚酸和类黄酮的测定方法。采用有机试剂萃取和超声破碎细胞的方法对烟草总酚酸和类黄酮进行提取，并通过高效液相色谱(HPLC)对烟草中6种植物酚酸和5种植物类黄酮进行了定量分析。结果显示：有机萃取结合超声破碎细胞的方法能够将酚酸和类黄酮从植物中有效分离；高效液相色谱(HPLC)能够将6种植物酚酸和5种植物类黄酮一次分离；HPLC测定香豆酸、阿魏酸、芥子酸、对甲氧基苯甲酸和肉桂酸的保留时间分别为18.11、20.03、20.51、20.76、22.86、26.23 min；HPLC测定杨梅素、槲皮素、根皮素、柑橘素、山奈酚的保留时间分别为9.06、12.01、14.96、15.23、16.12 min。通过研究表明，HPLC能够很好的对植物酚酸和类黄酮进行检测，这为研究木质素代谢途径相关酚酸和类黄酮奠定了基础。
　　위료연구목질소대사도경중상관분산화류황동적측정방법。채용유궤시제췌취화초성파쇄세포적방법대연초총분산화류황동진행제취，병통과고효액상색보(HPLC)대연초중6충식물분산화5충식물류황동진행료정량분석。결과현시：유궤췌취결합초성파쇄세포적방법능구장분산화류황동종식물중유효분리；고효액상색보(HPLC)능구장6충식물분산화5충식물류황동일차분리；HPLC측정향두산、아위산、개자산、대갑양기분갑산화육계산적보류시간분별위18.11、20.03、20.51、20.76、22.86、26.23 min；HPLC측정양매소、곡피소、근피소、감귤소、산내분적보류시간분별위9.06、12.01、14.96、15.23、16.12 min。통과연구표명，HPLC능구흔호적대식물분산화류황동진행검측，저위연구목질소대사도경상관분산화류황동전정료기출。
The aim was to establish a research method to measure the phenolic and flavonoid which relate to plant lignin biosynthesis. Organic reagent and ultrasonication were used for plant phenolic and flavonoid extract, HPLC was used for the measurement of six plant phenolic and five flavonoids. Tobacco was used as sample to test the HPLC measure method. The result showed that HPLC method for the phenolic and flavonoid relate to plant lignin biosynthesis was efficient which could isolate 6 different phenolics and 5 different flavonoids in one test;organic reagent and ultrasonication were useful to extract plant phenolic and flavonoid;the retention time of p-coumaric, ferulic, caffeic, cinnamic, sinapic and 4-methoxybenzoic acid were 18.11, 20.03, 20.51, 20.76, 22.86 and 26.23 min;the retention time of myricetin, quercetin, phloretin, naringenin and kaempferol were 9.06, 12.01, 14.96, 15.23, 16.12 min. The method made foundation for the research of plant lignin biosynthesis.