中华耳科学杂志
中華耳科學雜誌
중화이과학잡지
CHINESE JOURNAL OF OTOLOGY
2013年
2期
293-295
,共3页
王刚%吴玮%韩浩伦%屈昌北%王方园%王鸿南%李保卫%孟令照%虞学军%吴大蔚%牛聪敏%刘钢
王剛%吳瑋%韓浩倫%屈昌北%王方園%王鴻南%李保衛%孟令照%虞學軍%吳大蔚%牛聰敏%劉鋼
왕강%오위%한호륜%굴창북%왕방완%왕홍남%리보위%맹령조%우학군%오대위%우총민%류강
模拟失重%噪声%雌激素%听性脑干反应
模擬失重%譟聲%雌激素%聽性腦榦反應
모의실중%조성%자격소%은성뇌간반응
Simulated weightlessness%Noise%Estrogen%Auditory brainstem response
目的探讨模拟失重和飞船舱内噪声复合因素对豚鼠耳蜗听性脑干反应(ABR)阈值的影响及雌激素的防护作用。方法40只豚鼠随机分为单纯失重组、失重+噪声组、雌激素治疗组、雌激素预防组各10只。后肢悬吊法模拟失重，除单纯失重组外均暴露于模拟飞船舱内在天飞行及返回段的噪声环境，共5天。雌激素预防组实验前3天每天肌注苯甲酸雌二醇0.08 mg/kg，首剂加倍。雌激素治疗组实验中每天肌注苯甲酸雌二醇0.08 mg/kg，首剂加倍，持续至实验结束后3天。实验前、实验结束后即刻和实验结束后3天测试听性脑干反应阈值。结果各组实验前ABR阈值无差异，实验结束及恢复3天后各组ABR阈值之间有差异，雌激素治疗组ABR阈值最低。各组实验前后不同时间的ABR阈值均有差异。结论失重及失重+噪声均可造成豚鼠听功能损伤，后者造成的损伤更重。雌激素治疗对失重+噪声复合因素下的听功能损伤具有一定防护作用。
目的探討模擬失重和飛船艙內譟聲複閤因素對豚鼠耳蝸聽性腦榦反應(ABR)閾值的影響及雌激素的防護作用。方法40隻豚鼠隨機分為單純失重組、失重+譟聲組、雌激素治療組、雌激素預防組各10隻。後肢懸弔法模擬失重，除單純失重組外均暴露于模擬飛船艙內在天飛行及返迴段的譟聲環境，共5天。雌激素預防組實驗前3天每天肌註苯甲痠雌二醇0.08 mg/kg，首劑加倍。雌激素治療組實驗中每天肌註苯甲痠雌二醇0.08 mg/kg，首劑加倍，持續至實驗結束後3天。實驗前、實驗結束後即刻和實驗結束後3天測試聽性腦榦反應閾值。結果各組實驗前ABR閾值無差異，實驗結束及恢複3天後各組ABR閾值之間有差異，雌激素治療組ABR閾值最低。各組實驗前後不同時間的ABR閾值均有差異。結論失重及失重+譟聲均可造成豚鼠聽功能損傷，後者造成的損傷更重。雌激素治療對失重+譟聲複閤因素下的聽功能損傷具有一定防護作用。
목적탐토모의실중화비선창내조성복합인소대돈서이와은성뇌간반응(ABR)역치적영향급자격소적방호작용。방법40지돈서수궤분위단순실중조、실중+조성조、자격소치료조、자격소예방조각10지。후지현조법모의실중，제단순실중조외균폭로우모의비선창내재천비행급반회단적조성배경，공5천。자격소예방조실험전3천매천기주분갑산자이순0.08 mg/kg，수제가배。자격소치료조실험중매천기주분갑산자이순0.08 mg/kg，수제가배，지속지실험결속후3천。실험전、실험결속후즉각화실험결속후3천측시은성뇌간반응역치。결과각조실험전ABR역치무차이，실험결속급회복3천후각조ABR역치지간유차이，자격소치료조ABR역치최저。각조실험전후불동시간적ABR역치균유차이。결론실중급실중+조성균가조성돈서은공능손상，후자조성적손상경중。자격소치료대실중+조성복합인소하적은공능손상구유일정방호작용。
Objective To investigate the effects of combined simulated weightlessness and noise on auditory brainstem response thresholds in guinea pigs and the protective effects of estrogen. Methods Forty guinea pigs were randomly divided into a simulated weightlessness only group, a weightlessness with noise group, an estrogen treatment group and an estrogen prevention group. Weightlessness was simulated by posterior limb suspension, at a-30° angle between horizon and longitudi-nal axis of the body. Except for the weightlessness only group, animals in all other groups were also exposed to simulated spaceship flying and return stages noise for 5 days.Intramuscular injection of estradiol benzoate (0.08 mg/kg/day with dou-ble dose on Day 1) was administered for 3 days before experiment in the estrogen prevention group, and from the start of the experiment to 3 days after experiment in the estrogen treatment group. Auditory brainstem response (ABR) thresholds were re-corded before, at the end of and 3 days after the experiment. Results ABR thresholds were not different among the groups be-fore the experiment. ABR thresholds were different at the end of and 3 days after the experiment, with those in the estrogen treatment group being the lowest. ABR threshold differences were also seen at different times during experiment in each group. Conclusion While both weightlessness and noise can lead to damage of auditory function in guinea pigs, their combi-nation can cause even more severe damage. Estrogen appears to have protective effects against damage of auditory function caused by compound weightlessness and noise factors in space.
