CAJ | 학술논문

　　目的探讨不同浓度ouabain溶液对贵州小型猪内耳的作用，分析听力改变与药物浓度关系。方法采用健康、黑色2月龄贵州小型猪13只，随机分为实验组、对照组和正常对照组，其中试验组动物通过完整圆窗膜局部应用不同浓度ouabain，分为1mM组、0.1mM组，对照组动物局部使用等量生理盐水，正常对照组不予特殊处理。用药前后行听性脑干反应测定，并通过免疫荧光法，观察各组动物在应用药物后三天、一周、两周时的听功能及内耳形态学（螺旋神经节、毛细胞）的变化。结果生理盐水对照组及正常对照组内耳形态及听功能未见明显变化，不同浓度Ouabain均可导致螺旋神经节细胞数目减少，毛细胞形态及数量与对照组之间无显著性差异，ABR阈值变化明显(P<0.05)。结论经完整圆窗膜给药，ouabain溶液对小型猪螺旋神经节细胞有损伤，并且损伤程度具有浓度依赖性，并造成听阈明显提高，ouabain对毛细胞的影响不明显。
　　목적탐토불동농도ouabain용액대귀주소형저내이적작용，분석은력개변여약물농도관계。방법채용건강、흑색2월령귀주소형저13지，수궤분위실험조、대조조화정상대조조，기중시험조동물통과완정원창막국부응용불동농도ouabain，분위1mM조、0.1mM조，대조조동물국부사용등량생리염수，정상대조조불여특수처리。용약전후행은성뇌간반응측정，병통과면역형광법，관찰각조동물재응용약물후삼천、일주、량주시적은공능급내이형태학（라선신경절、모세포）적변화。결과생리염수대조조급정상대조조내이형태급은공능미견명현변화，불동농도Ouabain균가도치라선신경절세포수목감소，모세포형태급수량여대조조지간무현저성차이，ABR역치변화명현(P<0.05)。결론경완정원창막급약，ouabain용액대소형저라선신경절세포유손상，병차손상정도구유농도의뢰성，병조성은역명현제고，ouabain대모세포적영향불명현。
Objective To investigate the relationship among cochlear morphology, hearing changes and ouabain concen-tration. Method Thirteen healthy, 2-months-old black Guizhou miniature pigs were randomly divided into an experimental group, an experiment control group and a normal control group. Ouabain (200 ul at 1 mM or 0.1 mM) was delivered through in-tact round window membrane. The same volume of saline was administrated to the experiment control group animals through the same pathway. No treatment was applied to the normal control group. Auditory brainstem responses were measured before and after ouabain delivery. The number of spiral ganglion neurons was counted under a microscope. The morphology of hair cells in the organ of Corti was examined after the application of drugs for three days, one week or two weeks. Results There was no significant changes in the morphology of the inner ear and auditory function in the experiment control and normal con-trol groups. After application of ouabain, the number of spiral ganglion cells decreased and the ABR threshold increased signif-icantly (P<0.05). The number of hair cells showed no significant change. Conclusion The spiral ganglion neuron can be in-jured and hearing threshold elevated by ouabain delivered through intact round window membrane in a dose and time depen-dent manner. Hairs cells are not significantly affected by ouabain.