CAJ | 학술논문

　　对患病斑点叉尾鮰的肝脏病灶进行细菌分离培养，经 API20E 细菌生化快速试剂条鉴定为保科爱德华氏菌（Edwardsiella hoshinae）。人工感染试验可再现自然条件下鱼病的症状，表现出很强的致病性。病变组织切片观察发现，病变最初是部分肝细胞发生颗粒变性及水样变性，颗粒变性的肝细胞肿大。严重时，细胞周界不清晰，肝细胞内的小空泡连成大空泡，核悬浮在肝细胞的中央，细胞核肿大，并出现部分肝细胞的核浓缩、破裂、溶解消失。保科爱德华氏菌对斑点叉尾鮰具有显著致病性，可引起肝脏发生严重病变。
　　대환병반점차미회적간장병조진행세균분리배양，경 API20E 세균생화쾌속시제조감정위보과애덕화씨균（Edwardsiella hoshinae）。인공감염시험가재현자연조건하어병적증상，표현출흔강적치병성。병변조직절편관찰발현，병변최초시부분간세포발생과립변성급수양변성，과립변성적간세포종대。엄중시，세포주계불청석，간세포내적소공포련성대공포，핵현부재간세포적중앙，세포핵종대，병출현부분간세포적핵농축、파렬、용해소실。보과애덕화씨균대반점차미회구유현저치병성，가인기간장발생엄중병변。
Pathogenic bacteria strain was isolated from the livers of the diseased channel catfish. It was identified as Edwardsiella hoshinae by biochemical identification. Therefore artificial infection test indicated that the Edwardsiella hoshinae showed strong pathogenicity and caused the same symptoms as the naturally infected fish’s. As histopathological slices indicated, some liver cells showed granule degeneration and swollen, and some showed cellular hydropic degeneration at the beginning. Subsequently the cell membranes were illegible. Small vacuoles of liver cell merged into the bigger. Some cell nucleus suspended in the center, condensed, burst, dissolved and finally disappeared. These results demonstrated that Edwardsiella hoshinae had strong pathogenicity in channel catfish and could lead to pathological changes of liver.