CAJ | 학술논문

　　以脂肪质量分数分别为5.77%、7.71%、8.93%、11.89%、13.75%、16.42%的饲料饲喂红鳍东方鲀（Takifugu rubripe）幼鱼56 d，测定其肝脏抗氧化酶活力和总抗氧化能力，并对其肝脏、肠及肌肉进行组织学观察，研究不同饲料脂肪水平对红鳍东方鲀幼鱼肝脏抗氧化酶活力及组织结构的影响。结果表明，5.77%组过氧化氢酶（CAT）活力与其他各组间有显著性差异（P<0.05）。总抗氧化能力（T-AOC）活力的最高值出现在8.93%组，且显著高于其他各组（P<0.05）。饲料脂肪水平大于8.93%的实验组间超氧化物歧化酶（SOD）活力差异不显著（P>0.05），且显著低于8.93%组；5.77%、7.71%、8.93%组呈增加趋势，且各组间差异显著（P <0.05）。谷胱甘肽过氧化物酶（GSH-PX）活力的峰值出现在13.75%组，且显著高于其他各组（P<0.05），总体呈先增加后降低的趋势。组织学观察表明，5.77%、7.71%、8.93%组，虽然肝脏也有脂肪空泡存在，但是肝脏、肠和肌肉组织结构完整，无明显病变现象；11.89%、13.75%、16.42%组的肝脏、肠和肌肉均有不同程度的病变现象，各组间差别明显。由此认为，脂肪水平为8.93%组，抗氧化酶活力最高，该脂肪水平下的饲料对红鳍东方鲀幼鱼肝脏代谢最有利，组织结构完整无病变。
　　이지방질량분수분별위5.77%、7.71%、8.93%、11.89%、13.75%、16.42%적사료사위홍기동방돈（Takifugu rubripe）유어56 d，측정기간장항양화매활력화총항양화능력，병대기간장、장급기육진행조직학관찰，연구불동사료지방수평대홍기동방돈유어간장항양화매활력급조직결구적영향。결과표명，5.77%조과양화경매（CAT）활력여기타각조간유현저성차이（P<0.05）。총항양화능력（T-AOC）활력적최고치출현재8.93%조，차현저고우기타각조（P<0.05）。사료지방수평대우8.93%적실험조간초양화물기화매（SOD）활력차이불현저（P>0.05），차현저저우8.93%조；5.77%、7.71%、8.93%조정증가추세，차각조간차이현저（P <0.05）。곡광감태과양화물매（GSH-PX）활력적봉치출현재13.75%조，차현저고우기타각조（P<0.05），총체정선증가후강저적추세。조직학관찰표명，5.77%、7.71%、8.93%조，수연간장야유지방공포존재，단시간장、장화기육조직결구완정，무명현병변현상；11.89%、13.75%、16.42%조적간장、장화기육균유불동정도적병변현상，각조간차별명현。유차인위，지방수평위8.93%조，항양화매활력최고，해지방수평하적사료대홍기동방돈유어간장대사최유리，조직결구완정무병변。
The juvenile Takifugu rubripes with weight of 7.71 ± 0.15 g were fed with six levels of dietary lipid (5.77%, 7.71%, 8.93%, 11.89%, 13.75%and 16.42%) for 56 days, and antioxidant activities (CAT, GSH-PX, SOD and T-AOC) and the histology of the liver, intestine and muscle were tested to examine the effects of dietary lipid on the T. rubrupes juveniles. The results showed that the CAT was improved significantly in the group of 5.77%dietary lipid, the peak value of GSH-PX was observed in the group of 13.75%dietary lipid, and the level of T-AOC in the group of 8.93%was the highest (p<0.05). The levels of SOD decreased significantly in the groups of 11.89%, 13.75% and 16.42% dietary lipid, while increased in the group of 5.77%, 7.71%and 8.93%. There exist lipid vacuoles in the liver tissues of all the six groups, but no pathological changes in the low-level groups of lipid (5.77%, 7.71% and 8.93%). In addition, pathological changes were detected in the intestine and muscle tissues of the high lipid level groups (11.89%, 13.75%and 16.42%). It was concluded that the antioxidant activities were the best and there were no pathological changes when the juvenile were fed with 8.93%dietary lipid.