CAJ | 학술논문

　　通过提取环境总16S rDNA和总16S rRNA分别构建DNA克隆文库和反转录cDNA文库，并进行克隆测序和序列分析，探讨湖光岩玛珥湖浮游细菌和浮游活性细菌的遗传多样性。结果表明：构建两个水层的4个克隆文库，即1 m和10 m水层总菌的16S rDNA文库和总活性菌的16S rcDNA文库，共获得413条序列，分属15门、32目、52科；1 m 水层的 rDNA 文库与 rcDNA 文库中 Verrucomicrobia 类群所占比例最大（36.8%和32.1%），其次为Alphaproteobacteria（15.1%和16%）和Betaproteobacteria（17.9%和16%）；在10 m水层的2个文库中，Alphaproteobacteria类群均占绝对优势，分别为63.5%（rDNA）和43.8%（rcDNA），其次为Verrucomicrobia（11.5%和14.3%），其他细菌类群包括Acidobacteria、Chloroflexi、Firmicutes、待定门OD1、Planctomycetes、Spirochetes和Deltaproteobacteria，比例在0.8%~3.8%之间；在科的水平上，SAR11为湖光岩玛珥湖最为丰富的类群，占总克隆数的26.9%，其中在10 m水层的比例高达51.5%。序列同源性分析表明，绝大多数序列（89.8%）来源于新种，其中19.6%的序列可能来自于新属，69%的序列可能来自于全新的科。湖光岩玛珥湖浮游细菌群落结构较为独特，有大量的浮游细菌种类尚未获得相应的纯培养物，蕴含着丰富而新颖的微生物资源。
　　통과제취배경총16S rDNA화총16S rRNA분별구건DNA극륭문고화반전록cDNA문고，병진행극륭측서화서렬분석，탐토호광암마이호부유세균화부유활성세균적유전다양성。결과표명：구건량개수층적4개극륭문고，즉1 m화10 m수층총균적16S rDNA문고화총활성균적16S rcDNA문고，공획득413조서렬，분속15문、32목、52과；1 m 수층적 rDNA 문고여 rcDNA 문고중 Verrucomicrobia 류군소점비례최대（36.8%화32.1%），기차위Alphaproteobacteria（15.1%화16%）화Betaproteobacteria（17.9%화16%）；재10 m수층적2개문고중，Alphaproteobacteria류군균점절대우세，분별위63.5%（rDNA）화43.8%（rcDNA），기차위Verrucomicrobia（11.5%화14.3%），기타세균류군포괄Acidobacteria、Chloroflexi、Firmicutes、대정문OD1、Planctomycetes、Spirochetes화Deltaproteobacteria，비례재0.8%~3.8%지간；재과적수평상，SAR11위호광암마이호최위봉부적류군，점총극륭수적26.9%，기중재10 m수층적비례고체51.5%。서렬동원성분석표명，절대다수서렬（89.8%）래원우신충，기중19.6%적서렬가능래자우신속，69%적서렬가능래자우전신적과。호광암마이호부유세균군락결구교위독특，유대량적부유세균충류상미획득상응적순배양물，온함착봉부이신영적미생물자원。
Huguangyan Maar Lake belongs to a unique type of volcanic lake. Little is known about the microbial diversity in Maar Lake. So it is important to study the bacterial diversity in the water body for protecting and developing Maar Lake microbial resources. Total DNA and RNA were extracted from planktonic microbial communities in the Huguangyan Maar Lake, and 16S rDNA and 16S rcDNA clone libraries were constructed, and a number of clones were sequenced with the aim to analyze the genetic diversity of total bacterioplankton and active planktonic bacteria. Four clone libraries were constructed from the community DNA and cDNA of the 1 m and 10 m layers of the Huguangyan Maar Lake with 413 sequences reported, which were further classified into 15 phyla, 32 orders, and 52 families. Within the 16S rDNA library and 16S rcDNA library of the 1m layer, Verrucomicrobia occupied the largest proportion (36.8% and 32.1%, respectively), followed by Alphaproteobacteria (15.1% and 16%, respectively) and Betaproteobacteria (17.9% and 16%,respectively). Alphaproteobacteria represented the most abundant taxa within the two libraries of the 10 m layer with the proportions of 63.5%(rDNA) and 43.8%(rcDNA), respectively, followed by Verrucomicrobia (11.5% and 14.3%). Other bacterial groups include Acidobacteria, Chloroflexi, Firmicutes, candidate phylum OD1, Planctomycetes, Spirochetes and Deltaproteobacteria, with a proportion of 0.8~3.8%documented. At the family level, SAR11 was the most abundant group in the bacterioplankton of the Huguangyan Maar Lake, accounting for 26.9%in term of the number of clones and reaching a maximum of 51.5%at the 10 m layer. Sequence similarity analysis shows that most of the sequence (89.8%) may originate from novel species, of which 19.6%may come from novel genera and 69% may be derived from novel families. The community structure of bacterioplankton in the Huguangyan Maar Lake is very unique in terms of both diversity and abundance of bacterial groups. A large number of 16S rDNA and rcDNA clones do not have corresponding pure cultures, suggesting that the Huguangyan Maar Lake is inhabited by many novel bacterial species and that more attentions should be paid to the protection of the microbial resources in worldwide Maar lakes.