化学研究
化學研究
화학연구
CHEMICAL RESEARCHES
2013年
3期
293-298
,共6页
朱金花%徐辉%东莹莹%李德亮%刘绣华
硃金花%徐輝%東瑩瑩%李德亮%劉繡華
주금화%서휘%동형형%리덕량%류수화
植物激素%电离常数%紫外分光光度法%电位滴定%测定
植物激素%電離常數%紫外分光光度法%電位滴定%測定
식물격소%전리상수%자외분광광도법%전위적정%측정
plant hormone%ionization constant%ultraviolet spectrophotometry%potentiometric titration%determination
分别采用紫外分光光度法和电位滴定法测定了脱落酸、3-吲哚乙酸、赤霉素、水杨酸等几种植物激素的电离常数,对比分析了两种测试方法的原理、测试步骤和数据处理方法。结果表明,采用两种方法测定的四种样品的电离常数与文献报道值一致,且测量结果的重现性较好;这表明紫外分光光度法和电位滴定法可方便地用于测定有机化合物的电离常数。
分彆採用紫外分光光度法和電位滴定法測定瞭脫落痠、3-吲哚乙痠、赤黴素、水楊痠等幾種植物激素的電離常數,對比分析瞭兩種測試方法的原理、測試步驟和數據處理方法。結果錶明,採用兩種方法測定的四種樣品的電離常數與文獻報道值一緻,且測量結果的重現性較好;這錶明紫外分光光度法和電位滴定法可方便地用于測定有機化閤物的電離常數。
분별채용자외분광광도법화전위적정법측정료탈락산、3-신타을산、적매소、수양산등궤충식물격소적전리상수,대비분석료량충측시방법적원리、측시보취화수거처리방법。결과표명,채용량충방법측정적사충양품적전리상수여문헌보도치일치,차측량결과적중현성교호;저표명자외분광광도법화전위적정법가방편지용우측정유궤화합물적전리상수。
Ultraviolet spectrophotometric method and potentiometric titration method were sep-arately adopted to determine the ionization constant of several plant hormones including abscisic acid ,3-indole acetic acid ,gibberellic acid and salicylic acid .T he principle ,procedure ,and da-ta-process of the two methods were discussed and compared .Results indicate that the ioniza-tion constants of the four samples determined by the two methods are in agreement with that reported in literatures ,and they have good reproducibilities .This means that both ultraviolet spectrophotometric method and potentiometric titration method can be well adopted to deter-mine the ionization constants of organic compounds .