CAJ | 학술논문

　　通过观察不同剂量二甲基亚砜( dimethyl sulfoxide DMSO)作用下PC12细胞形态和生长状态的变化，确定PC12细胞对DMSO的耐受剂量.按照一定浓度梯度（V/V）将DMSO加入培养液干预后的24、48和72 h于倒置相差显微镜下观察AO/EB染色前后细胞的形态特征，同时用四甲基偶氮唑蓝（MTT）显色法检测细胞的生存率和计算半数抑制浓度（50%inhibiting concentration，IC50）．结果表明：DMSO浓度为14 mL/L时，24 h内90%的PC12细胞生长正常，形态完整，活力与对照组比较无差别，浓度提高到20 mL/L时，24 h时细胞生存率可下降27%，随着作用时间延长，细胞生存率进一步降低；24、48和72 h对应的IC50值分别为31.4、23.9和19.2 mL/L．因此，对PC12细胞而言，24 h内的应用终浓度应低于14 mL/L，干预时间如需延长， DMSO的应用剂量应相应降低．
　　통과관찰불동제량이갑기아풍( dimethyl sulfoxide DMSO)작용하PC12세포형태화생장상태적변화，학정PC12세포대DMSO적내수제량.안조일정농도제도（V/V）장DMSO가입배양액간예후적24、48화72 h우도치상차현미경하관찰AO/EB염색전후세포적형태특정，동시용사갑기우담서람（MTT）현색법검측세포적생존솔화계산반수억제농도（50%inhibiting concentration，IC50）．결과표명：DMSO농도위14 mL/L시，24 h내90%적PC12세포생장정상，형태완정，활력여대조조비교무차별，농도제고도20 mL/L시，24 h시세포생존솔가하강27%，수착작용시간연장，세포생존솔진일보강저；24、48화72 h대응적IC50치분별위31.4、23.9화19.2 mL/L．인차，대PC12세포이언，24 h내적응용종농도응저우14 mL/L，간예시간여수연장， DMSO적응용제량응상응강저．
The morphologic changes and growth status of PC12 cell were observed after being intervened by different concentrations of Dimethyl Sulfoxide and the safe concentrations were ascertained. AO/PI staining was chosen to show the morphologic changes of PC12 and cellular pictures were taken before and after being stained when cells were cultured full of 24、48 and 72 h respectively. At the same time, cell viability and IC50 were quantitated based on the assay of MTT. Except for regular growth, 90%of the PC12 also could keep per?fect structure and the same cell viability as the control in 24 h if the concentration of DMSO was 14 mL/L. If the concentration of DMSO was up to 20 mL/L , the cell viability would be down to 73%in 24 h. The longer the time, the more decreased the percentages of cell viability. The IC50 values corresponding to 24、48 and 72 h were 31.4、23.9 and 19.2 mL/L respectively. The safe concentration of DMSO to PC12 was not above 14 mL/L in 24 hours, if needing extension of intervention time, the corresponding concentrations of DMSO should be re?duced.