CAJ | 학술논문

　　应用酶解去壁法制备蝴蝶兰染色体标本，对供试材料的选取、预处理方法、酶解时间和温度加以探索．实验结果显示，换盆后14～20 d的盆苗嫩根根尖分裂活性最高、中期分裂相较多．剪取长1 cm左右的根尖用0.002M的8-羟基喹啉在18℃预处理4 h，卡诺固定液固定2～24 h，再用4%纤维素酶和1%果胶酶的混合酶液在25℃下酶解1.5 h，能获得高质量的制片，染色体在载玻片上分散良好、形态清晰.
　　응용매해거벽법제비호접란염색체표본，대공시재료적선취、예처리방법、매해시간화온도가이탐색．실험결과현시，환분후14～20 d적분묘눈근근첨분렬활성최고、중기분렬상교다．전취장1 cm좌우적근첨용0.002M적8-간기규람재18℃예처리4 h，잡낙고정액고정2～24 h，재용4%섬유소매화1%과효매적혼합매액재25℃하매해1.5 h，능획득고질량적제편，염색체재재파편상분산량호、형태청석.
By using shedding cell wall with enzyme to make the picture of karyotype of Phalaenopsis, We explored on the activity of plant materials、pretreatment、time and temperature of incubated in enzyme. The result indicated that the young root tips of Phalaenopsis after changing pot for 14~20 d are the best materials for experiment. The best preparation procedures are as follows:1cm long root tips were immersed in 0.002 M 8-hydroxyquinolin for 4h at 18℃,fixed in Carnoy’s for 4~20 h at 4℃,incubated in a mixture of 4%cellu?lose and 1%pectolyase for 1.5 h at 25℃. The result showed that there were lots of scattering cell on the pic?tures. It was optimum for chromosome preparation because it allowed morphological characteristics to become visible.