CAJ | 학술논문

　　目的研究格尔德霉素对人肝癌HepG2细胞增殖及凋亡的影响。方法不同浓度格尔德霉素与人肝癌HepG2细胞共培养，采用MTT法检测增殖抑制率；采用流式细胞术Annexin V法检测细胞凋亡率。结果在0.2、0.4和0.8μmol/L浓度下，格尔德霉素均显著抑制HepG2细胞的增殖，在干预细胞48h后，其抑制率分别为20.36%、29.45%和42.52%，抑制率随药物浓度增加而升高；同时，细胞凋亡率分别为4.82%、24.47%和53.64%，也呈剂量依赖性，与相应对照组比，差异有统计学意义（P＜0.05）。结论格尔德霉素可抑制肝癌HepG2细胞增殖并诱导其调亡。
　　목적연구격이덕매소대인간암HepG2세포증식급조망적영향。방법불동농도격이덕매소여인간암HepG2세포공배양，채용MTT법검측증식억제솔；채용류식세포술Annexin V법검측세포조망솔。결과재0.2、0.4화0.8μmol/L농도하，격이덕매소균현저억제HepG2세포적증식，재간예세포48h후，기억제솔분별위20.36%、29.45%화42.52%，억제솔수약물농도증가이승고；동시，세포조망솔분별위4.82%、24.47%화53.64%，야정제량의뢰성，여상응대조조비，차이유통계학의의（P＜0.05）。결론격이덕매소가억제간암HepG2세포증식병유도기조망。
Objective To investigate the effects of geldanamycin on the proliferation and apoptosis in hu-man hepatoma cell lines HepG2. Methods HepG2 cells were incubated with different concentrations of gel-danamycin for 24 to 72 hours. Cell proliferation was determined by MTT assay. Cell apoptosis was detected by flow cytometry using annexin V and propidium iodide. Results Geldanamycin significantly inhibited the prolifera-tion of HepG2 cells at a dose-dependent manner. When compared to the control,the cell proliferation was inhibit-ed by 20.36%,29.45% and 42.52%,respectively after 48h incubation at the concentration of 0.2,0.4 and 0.8μmol/L of geldanamycin,however,the apoptosis of HepG2 cells was increased by 4.82%,24.47% and 53.64%(P<0.05). Conclusions Geldanamycin inhibits proliferation and induces apoptosis in HepG2 cells,suggesting a therapeutic effect of geldanamycin in hepatoma treatment.