CAJ | 학술논문

　　目的探讨中药肝复康对大鼠肝纤维化组织PI3K/PKB信号通路和血小板衍生生长因子受体-β（PDGFR-β）表达的影响。方法制备大鼠四氯化碳诱导的肝纤维化模型，给予肝复康干预。采用免疫组织化学法检测肝组织PDGFR-β蛋白的表达，采用半定量RT-PCR法测定肝组织PI3K和PKB1mRNA水平的变化。结果与对照组比，模型组肝组织PDGFR-β蛋白的表达上调，而肝复康治疗组其表达被显著抑制；模型组肝组织PI3K和PKB1 mRNA水平的相对光密度值分别为2.08±0.09和2.54±0.10，较对照组均显著上升（0.86±0.04和0.36±0.03， P<0.01），但肝复康治疗组其水平均被显著抑制（分别为0.88±0.05和0.41±0.03，与模型组比，P<0.01）。结论肝复康对肝纤维化的治疗作用可能与其作用于PI3K/PKB信号转导途径和抑制PDGFR-β表达有关。
　　목적탐토중약간복강대대서간섬유화조직PI3K/PKB신호통로화혈소판연생생장인자수체-β（PDGFR-β）표체적영향。방법제비대서사록화탄유도적간섬유화모형，급여간복강간예。채용면역조직화학법검측간조직PDGFR-β단백적표체，채용반정량RT-PCR법측정간조직PI3K화PKB1mRNA수평적변화。결과여대조조비，모형조간조직PDGFR-β단백적표체상조，이간복강치료조기표체피현저억제；모형조간조직PI3K화PKB1 mRNA수평적상대광밀도치분별위2.08±0.09화2.54±0.10，교대조조균현저상승（0.86±0.04화0.36±0.03， P<0.01），단간복강치료조기수평균피현저억제（분별위0.88±0.05화0.41±0.03，여모형조비，P<0.01）。결론간복강대간섬유화적치료작용가능여기작용우PI3K/PKB신호전도도경화억제PDGFR-β표체유관。
Objective To investigate the effects of traditional Chinese herbal medicine Gan-fu-kang on hepatic PI3K/PKB signaling pathway and platelet-derived growth factor-β(PDGFR-β) in fibrotic liver in rats. Methods A rat model of liver fibrosis were induced by injection of 10%carbon tetrachloride (CCl4) subcuta-neously and was treated with Gan-fu-kang. The PDGFR-β protein in livers was detected by immunohistochem-istry and the PI3K and PKB1 mRNA were detected by semi-quantitive RT-PCR. Results The hepatic PDGFR-β protein in model animals was markedly increased than in the controls and was suppressed by Gan-fu-kang treatment;In the controls,the relative quantification value of PI3K and PKB1 mRNA were 0.86±0.04 and 0.36±0.03, respectively,which were significantly lower than that in the model animals (2.08±0.09 and 2.54±0.10,respectively,P<0.01); However,Gan-fu-kang treatment significantly inhibited the increased PI3K and PKB1 mRNA levels(0.88±0.05 and 0.41 ±0.03,respectively,P<0.01) as compared to that in the models. Conclusion Gan-fu-kang may inhibit liver fibrosis by through inhibition of PI3K/PKB signaling pathway and PDGFR-β expression.