CAJ | 학술논문

　　采用索氏回流提取法提取三尖杉内生真菌SR1－26（Fusarium sp．）的挥发油，利用气相色谱－质谱联用技术（GC－MS）分离和鉴定其化学成分，并通过滤纸片扩散法初步研究挥发油的抑菌活性。从内生真菌 SR1－26的挥发油中共鉴定出18种化学成分，主要为油酸（58．61％）、棕榈酸（10．06％）、油酸乙酯（12．05％）等脂肪酸类及部分脂肪烷烃和烯醛类物质；活性检测表明挥发油样品对金黄色葡萄球菌（Staphylococcus aureus）、大肠杆菌（Escherichia coli）和沙门氏菌（Salmonella typhl）显示较强的抑制作用。
　　채용색씨회류제취법제취삼첨삼내생진균SR1－26（Fusarium sp．）적휘발유，이용기상색보－질보련용기술（GC－MS）분리화감정기화학성분，병통과려지편확산법초보연구휘발유적억균활성。종내생진균 SR1－26적휘발유중공감정출18충화학성분，주요위유산（58．61％）、종려산（10．06％）、유산을지（12．05％）등지방산류급부분지방완경화희철류물질；활성검측표명휘발유양품대금황색포도구균（Staphylococcus aureus）、대장간균（Escherichia coli）화사문씨균（Salmonella typhl）현시교강적억제작용。
The volatile oils of endophytic fungus SR1-26 isolated from Cephalotaxus fortunei were ex-tracted with Soxhlet extraction method.The chemical composition were separated and identified by gas chroma-tography-mass spectrometry(GC-MS).We studied the antimicrobial activity of the volatile oils by filter paper diffusion method.The volatiles were mainly composed of 18 components in the mycelium of endophytic fungus SR1-26:oleic acid (58.61%), palmitic acid (10.06%), linnleic acid, ethyl oleate (12.05%), these ali-phatic acids, alkanes and aldehydes, and so on.The volatiles exhibited antimicrobial activities against Staphy-lococcus aureus, Escherichia coli and Salmonella typhl .