CAJ | 학술논문

　　以（NH4）2 SO4为唯一氮源，经富集培养、平板涂布分离及平板划线纯化、摇瓶复筛等方法，从当地养殖池水体中分离、筛选氨氮降解菌。根据形态学、生理生化特征分析，对其进行归属鉴定，并研究其降解特性。通过对初步鉴定归属为沙雷氏菌属（Serratia ．sp），命名为N-2的氨氮降解菌降解特性的研究，得到其最适降解条件：N -2利用（NH4）2 SO4作唯一氮源时，最适碳源为蔗糖，降解温度为32～37℃，pH为7．0。在最适条件下，当NH 4-N初始质量浓度为50mg/L时，该菌培养至＋72h氨氮降解率可达97．4％。结果表明，该菌为高效氨氮降解菌，这种土著微生物在修复富营养化水体方面有较好的应用前景。
　　이（NH4）2 SO4위유일담원，경부집배양、평판도포분리급평판화선순화、요병복사등방법，종당지양식지수체중분리、사선안담강해균。근거형태학、생리생화특정분석，대기진행귀속감정，병연구기강해특성。통과대초보감정귀속위사뢰씨균속（Serratia ．sp），명명위N-2적안담강해균강해특성적연구，득도기최괄강해조건：N -2이용（NH4）2 SO4작유일담원시，최괄탄원위자당，강해온도위32～37℃，pH위7．0。재최괄조건하，당NH 4-N초시질량농도위50mg/L시，해균배양지＋72h안담강해솔가체97．4％。결과표명，해균위고효안담강해균，저충토저미생물재수복부영양화수체방면유교호적응용전경。
To screen indigenous microorganisms of purificating aquaculture water ,a high effective strain N -2 degrading ammonia-nitrogen has been isolated from the local breeding pond water with (NH4 )2 SO4 as sole nitrogen source via enrichment culture ,spread plate separation and streak plate purification steps .N-2 was identified as Serratia .sp by the morphology ,physiological and biochemical characteristics . The optimal conditions for growth and degradation as follows :32~37℃ ,pH 7 .0 ,sucrose as carbon source .The degrading rate of NH+4 -N by N-2 was over 97 .4% within 72 h at initial concentration of NH 4 -N was 50 mg/L .It indicated that N-2 strain has good applica-+tion prospects in eutrophic water remediation .